Trade‑offs and timing of leaf defenses during development in co‑occurring evergreen and deciduous Quercus

Sancho Knapik, DomingoUrcola Lázaro, NereaAlonso Forn, DavidMartín Sánchez, RubénPeguero Pina, José JavierFerrio Díaz, Juan PedroGil Pelegrín, Eustaquio2026-02-262026-02-262026-02-19Sancho-Knapik, D., Urcola-Lázaro, N., Alonso‑Forn, D., Martín‑Sánchez, R., Peguero‑Pina, J. J., Ferrio, J. P., & Gil‑Pelegrín, E. (2026). Trade‑offs and timing of leaf defenses during development in co‑occurring evergreen and deciduous Quercus. Research Square. https://doi.org/10.21203/rs.3.rs-8639643/v1https://doi.org/10.21203/rs.3.rs-8639643/v1https://hdl.handle.net/10532/8151Context: Young, expanding leaves are nutrient-rich and mechanically weak, making them highly attractive to herbivores. How defense timing and allocation differ during development between co-occurring evergreen and deciduous species remains unclear. Aims: We tested whether evergreen oaks invest more in direct defenses during leaf development than deciduous oaks, and whether each habit prioritizes distinct defensive traits across stages. Methods: In a Mediterranean common garden, we monitored co-occurring evergreen Quercus rotundifoliaand deciduous Q. faginea from budburst to leaf maturity, measuring growth and defense traits. Seasonal trajectories and toughness–tannin relationships were analyzed, and patterns validated with three additional evergreen and three deciduous congeners. Results: Leaf area reached its maximum before LMA, creating a juvenile phase with low fibre content and toughness. The evergreen oak increased condensed tannins concentration early, then work of fracture; the deciduous oak grew faster initially, then reinforced mechanically and delayed tannins. Across stages, the evergreen kept higher work of fracture and condensed tannins, while the deciduous prioritized early growth; defense trade-off patterns were habit-specific and consistent across species. Conclusion: Leaf-habit–specific defense schedules shape protection: evergreens front-load chemical defenses before structural hardening, while deciduous oaks prioritize tolerance and earlier mechanical reinforcement, patterns with potential implications for herbivore pressure and associational dynamics in mixed stands.enAttribution-NonCommercial-NoDerivatives 4.0 InternationalTrade‑offs and timing of leaf defenses during development in co‑occurring evergreen and deciduous Quercusartículo preliminar2026-02-2310.21203/rs.3.rs-8639643/v1TaninoReacción bioquímicaTasa de crecimientoHojaQuercusFracturaVida de ecosistemas terrestres