Torres Punina, Andrea de las MercedesGuerra Velo, Mª EngraciaRodrigo García, Francisco JavierHedhly, Afif2026-01-282026-01-282026-01-14Torres, A., Guerra, M. E., Rodrigo, J., & Hedhly, A. (2026). New methodology for genomic characterization of S-alleles in Japanese plum cultivars. En: Jornada Investigadores Predoctorales IA2 - Campus Aula Dei 2026 (Zaragoza. España. 2026)https://hdl.handle.net/10532/8117Prunus species have a gametophytic incompatibility system that prevents self-fertilization and promotes crosspollination. This system is regulated by the multiallelic S locus, formed by two closely linked genes, the S-RNase expressed in the pistil and the SFB in the pollen tube. The identification of both S alleles of a cultivar is essential to establish its pollination needs, as well as its suitability as a parent in breeding programs. To date, in Japanese plum (hybrids of Prunus salicina Lindl.), 57 S alleles have been described (42 S-RNase and 15 SFB), which has allowed the assignment of more than 200 cultivars to 26 incompatibility groups. However, identification of S alleles has not been possible in a number of cultivars, presumably because their S alleles lack length polymorphism with already known alleles and have not been previously sequenced. In this work, we resequenced the genome of nine Japanese plum cultivars and applied a novel NGS-based methodology for S-genotyping and targeted de novo assembly of S-alleles and loci.enAttribution-NonCommercial-NoDerivs 4.0 Spaintexto2026-01-27Prunus salicinaAleloIncompatibilidad del polenGenómicaHambre cero