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dc.contributor.authorMuñoz Alvaro, Pilar Maríaes_ES
dc.contributor.authorBoadella, Marianaes_ES
dc.contributor.authorArnal Barrera, Maricruzes_ES
dc.contributor.authorMiguel López, María Jesús dees_ES
dc.contributor.authorRevilla Calavia, Migueles_ES
dc.contributor.authorMartínez, Davides_ES
dc.contributor.authorVicente Baños, Joaquínes_ES
dc.contributor.authorAcevero Lavandera, Pelayoes_ES
dc.contributor.authorOleaga Ruiz de Escudero, Alvaroes_ES
dc.contributor.authorRuiz Fons, José Franciscoes_ES
dc.contributor.authorMarín Alcalá, Clara Maríaes_ES
dc.contributor.authorPrieto Martín, José Migueles_ES
dc.contributor.authorFuente García, José de Jesús de laes_ES
dc.contributor.authorBarral, Martaes_ES
dc.contributor.authorBarberán Pelegrín, Montserrates_ES
dc.contributor.authorFernández de Luco Martínez, Danieles_ES
dc.contributor.authorBlasco Martínez, José Maríaes_ES
dc.contributor.authorGortázar Schmidt, Christianes_ES
dc.identifier.citationMuñoz, Pilar M.; Boadella, Mariana; Arnal, Maricuz; Miguel, María J. de; Revilla, Miguel; Marínez, David; Vicente, Joaquín; Acevedo, Pelayo; Oleaga, Alvaro; Ruiz-Fons, Francisco; Marín, Clara M.; Prieto, José M.; Fuente, José de la; Barral, Marta; Barberán, Montserrat; Fernández de Luco, Daniel; Blasco, José M.; Gortázar, Christian. "Spatial distribution and risk factors of Brucellosis" BMC Infectious diseases. 2010, 10:46, p. 1-14es_ES
dc.description.abstractBackground: The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates. Methods: A multi-species indirect immunosorbent assay (iELISA) using Brucella S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (Sus scrofa), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs. Results: Mean apparent prevalence below 0.5% was identified in chamois (Rupicapra pyrenaica), Iberian wild goat (Capra pyrenaica), and red deer (Cervus elaphus). Roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis aries) and Barbary sheep (Ammotragus lervia) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating B. abortus biovar 1 and B. melitensis biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as B. suis biovar 2. DNA polymorphisms were similar to those found in domestic pigs. Conclusions: In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.es_ES
dc.subject.otherPenínsula Ibéricaes_ES
dc.subject.otherProducción y sanidad animales_ES
dc.titleSpatial distribution and risk factors of Brucellosises_ES
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