Please use this identifier to cite or link to this item: http://hdl.handle.net/10532/1862
Title: The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice
Authors: San Román, Beatriz
Garrido, Victoria
Muñoz Alvaro, Pilar María
Arribillaga, Laura
García, Begoña
Andrés, Ximena de
Zabaleta, Virginia
Mansilla, Cristina
Farrán, Inmaculada
Lasa, Iñigo
Andrés, Damián de
Amorena Zabalza, Beatriz
Lasarte, Juan José
Grilló Dolset, María Jesús
Issue Date: 2012
Citation: Beatriz San Roman, Victoria Garrido, Pilar-Maria Munoz, Laura Arribillaga, Begona Garcia, Ximena De Andres, Virginia Zabaleta, Cristina Mansilla, Inmaculada Farran, Inigo Lasa, Damian De Andres, Beatriz Amorena, Juan-Jose Lasarte and Maria-Jesus Grillo. “The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice”. Veterinary Research 2012, 43:31 
Abstract: The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking OPS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SEΔwaaL) or deep-defective (SEΔgal) LPSCore, were used in combination with EDA. Biotinylated bacterins, in particular SEΔwaaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-polysaccharide and the usefulness of BEDA-SEΔwaaL as non-live vaccine in the mouse model.
URI: http://hdl.handle.net/10532/1862
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