Please use this identifier to cite or link to this item: http://hdl.handle.net/10532/2021
Title: Validación de genes de referencia normalizadotes de la expresión génica para la respuesta a estrés térmico en la especie ovina
Other Titles: Detection of reference genes for the heat stress response in the ovine species
Authors: Serrano, Mª Magdalena
González, Carmen
Moreno Sánchez, Natalia
Marcos Carcavilla, Ane
Van Poucke, Mario
Calvo Lacosta, Jorge Hugo
Salces Ortiz, Judit
Carabaño, María J.
Issue Date: 2011
Citation: Serrano, M., González, C., Moreno-Sánchez, N., Marcos-Carcavilla, A., Van Poucke, M., Calvo, J.H., Salces, J., y Carabaño, M.J. “Validación de genes de referencia normalizadotes de la expresión génica para la respuesta a estrés térmico en la especie ovina”. En: 41 Jornadas de Estudio. XIV Jornadas sobre Producción Animal. AIDA. Zaragoza, España, 17 y 18 de mayo de 2011, pp.497-499. ISBN: 978-84-615-0062-8
Abstract: We have tested 16 putative reference genes in peripheral whole blood of control and heat stressed ovine samples to detect stable genes for the heat-shock response. To determine gene expression stability a new approach based in Maximum Likelihood estimation of Mixed Model parameters has been tested and compared to commonly used software (NormFinder and geNorm). Differences in the stability rankings of genes were found among the three approaches. Under our approach SDHA and MDH1 was the best set of genes to be normalizers of target genes in heat shock experiments in the ovine specie
URI: http://hdl.handle.net/10532/2021
Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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