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Title: S-genotyping in Japanese plum by PCR and capillary gel electrophoresis detection
Authors: Guerra Velo, Mª Engracia
López Corrales, Margarita
Rodrigo García, Francisco Javier
Wünsch, Ana
Issue Date: 2012
Citation: Guerra , M.E., López-Corrales, M., Rodrigo , J. and Wünsch, A. (2012). “S-genotyping in Japanese plum by PCR and capillary gel electrophoresis detection” XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Plant Physiology from Cell to Fruit Production System. Lisbon, Portugal, August 22-27, 2010. Acta Hort. (ISHS) 932:139-142
Abstract: In this work a PCR S-genotyping method using capillary electrophoresis detection was assayed in Japanese plum. Sweet cherry primers designed for S-RNase and SFB intron length polymorphism detection by capillary electrophoresis were evaluated in Japanese plum cultivars. Amplification of both genes was successful and amplified sizes were correlated with Japanese plum S-alleles. The S-RNase genotype of 58 Japanese plum type cultivars previously determined by other methods was confirmed using this technology and the SFB alleles of these cultivars were also determined. Allele sizes of both genes are reported for 13 different S-alleles found in Japanese plum and will allow efficient S-genotype characterization in this species.
ISSN: 0567-7572
Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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