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dc.contributor.authorPérez Etayo, Laraes_ES
dc.contributor.authorMiguel López, María Jesús dees_ES
dc.contributor.authorConde Alvarez, Raqueles_ES
dc.contributor.authorMuñoz Alvaro, Pilar Maríaes_ES
dc.contributor.authorKhames, Mammares_ES
dc.contributor.authorIriarte, Maitees_ES
dc.contributor.authorMoriyón Uria, Ignacioes_ES
dc.contributor.authorZúñiga Ripa, Amaiaes_ES
dc.coverage.spatialProducción y sanidad animales_ES
dc.identifier.citationVeterinary Research, vol. 49, núm. 1 (2018)-
dc.description.abstractBrucella bacteria cause brucellosis, a major zoonosis whose control requires efficient diagnosis and vaccines. Identification of classical Brucella spp. has traditionally relied on phenotypic characterization, including surface antigens and 5–10% CO2 necessity for growth (CO2-dependence), a trait of Brucella ovis and most Brucella abortus biovars 1–4 strains. Although molecular tests are replacing phenotypic methods, CO2-dependence remains of interest as it conditions isolation and propagation and reflects Brucella metabolism, an area of active research. Here, we investigated the connection of CO2-dependence and carbonic anhydrases (CA), the enzymes catalyzing the hydration of CO2 to the bicarbonate used by anaplerotic and biosynthetic carboxylases. Based on the previous demonstration that B. suis carries two functional CAs (CAI and CAII), we analyzed the CA sequences of CO2-dependent and -independent brucellae and spontaneous mutants. The comparisons strongly suggested that CAII is not functional in CO2-dependent B. abortus and B. ovis, and that a modified CAII sequence explains the CO2-independent phenotype of spontaneous mutants. Then, by mutagenesis and heterologous plasmid complementation and chromosomal insertion we proved that CAI alone is enough to support CO2-independent growth of B. suis in rich media but not of B. abortus in rich media or B. suis in minimal media. Finally, we also found that insertion of a heterologous active CAII into B. ovis reverted the CO2-dependence but did not alter its virulence in the mouse model. These results allow a better understanding of central aspects of Brucella metabolism and, in the case of B. ovis, provide tools for large-scale production of diagnostic antigens and vaccines.en
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.titleThe CO2-dependence of Brucella ovis and Brucella abortus biovars is caused by defective carbonic anhydrasesen
dc.typeJournal Contribution*
dc.bibliographicCitation.titleVeterinary Researchen
Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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