Please use this identifier to cite or link to this item: http://hdl.handle.net/10532/5069
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dc.contributor.authorAragón Aranda, Beatrizes_ES
dc.contributor.authorMiguel López, María Jesús dees_ES
dc.contributor.authorLázaro Antón, Leticiaes_ES
dc.contributor.authorSalvador Bescós, Miriames_ES
dc.contributor.authorZúñiga Ripa, Amaiaes_ES
dc.contributor.authorMoriyón Uria, Ignacioes_ES
dc.contributor.authorIriarte, Maitees_ES
dc.contributor.authorMuñoz Alvaro, Pilar Maríaes_ES
dc.contributor.authorConde Alvarez, Raqueles_ES
dc.coverage.spatialProducción y sanidad animales_ES
dc.date.accessioned2020-09-10T08:31:10Z-
dc.date.available2020-09-10T08:31:10Z-
dc.date.issued2020es_ES
dc.identifier.citationVeterinary Research, vol. 51, num. 1, (2020)-
dc.identifier.urihttp://hdl.handle.net/10532/5069-
dc.description.abstractBrucella is a genus of gram-negative bacteria that cause brucellosis. B. abortus and B. melitensis infect domestic ruminants while B. suis (biovars 1-3) infect swine, and all these bacteria but B. suis biovar 2 are zoonotic. Live attenuated B. abortus S19 and B. melitensis Rev1 are effective vaccines in domestic ruminants, though both can infect humans. However, there is no swine brucellosis vaccine. Here, we investigated the potential use as vaccines of B. suis biovar 2 rough (R) lipopolysaccharide (LPS) mutants totally lacking O-chain (Bs2ΔwbkF) or only producing internal O-chain precursors (Bs2Δwzm) and mutants with a smooth (S) LPS defective in the core lateral branch (Bs2ΔwadB and Bs2ΔwadD). We also investigated mutants in the pyruvate phosphate dikinase (Bs2ΔppdK) and phosphoenolpyruvate carboxykinase (Bs2ΔpckA) genes encoding enzymes bridging phosphoenolpyruvate and the tricarboxylic acid cycle. When tested in the OIE mouse model at the recommended R or S vaccine doses (108 and 105 CFU, respectively), CFU/spleen of all LPS mutants were reduced with respect to the wild type and decreased faster for the R than for the S mutants. At those doses, protection against B. suis was similar for Bs2ΔwbkF, Bs2Δwzm, Bs2ΔwadB and the Rev1 control (105 CFU). As described before for B. abortus, B. suis biovar 2 carried a disabled pckA so that a double mutant Bs2ΔppdKΔpckA had the same metabolic phenotype as Bs2ΔppdK and ppdK mutation was enough to generate attenuation. At 105 CFU, Bs2ΔppdK also conferred the same protection as Rev1. As compared to other B. suis vaccine candidates described before, the mutants described here simultaneously carry irreversible deletions easy to identify as vaccine markers, lack antibiotic-resistance markers and were obtained in a non-zoonotic background. Since R vaccines should not elicit antibodies to the S-LPS and wzm mutants carry immunogenic O-chain precursors and did not improve Bs2ΔwbkF, the latter seems a better R vaccine candidate than Bs2Δwzm. However, taking into account that all R vaccines interfere in ELISA and other widely used assays, whether Bs2ΔwbkF is advantageous over Bs2ΔwadB or Bs2ΔppdK requires experiments in the natural host.en
dc.language.isoenes_ES
dc.relation.urihttps://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-020-00815-8es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleDevelopment of attenuated live vaccine candidates against swine brucellosis in a non-zoonotic B. suis biovar 2 backgrounden
dc.typeJournal Contribution*
dc.bibliographicCitation.volume51(1)es_ES
dc.subject.agrovocBrucella suises
dc.subject.agrovocBrucelosises
dc.subject.agrovocVacunaes
dc.subject.agrovocVacuna vivaes
dc.description.statusPublishedes_ES
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES
dc.bibliographicCitation.titleVeterinary Researchen
dc.relation.doi10.1186/s13567-020-00815-8es_ES
Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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