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http://hdl.handle.net/10532/2021| Title: | Validación de genes de referencia normalizadotes de la expresión génica para la respuesta a estrés térmico en la especie ovina |
| Other Titles: | Detection of reference genes for the heat stress response in the ovine species |
| Authors: | Serrano, Mª Magdalena González, Carmen Moreno Sánchez, Natalia Marcos Carcavilla, Ane Van Poucke, Mario Calvo Lacosta, Jorge Hugo Salces Ortiz, Judit Carabaño Luengo, María Jesús |
| Issue Date: | 2011 |
| Citation: | Serrano, M., González, C., Moreno-Sánchez, N., Marcos-Carcavilla, A., Van Poucke, M., Calvo, J.H., Salces, J., y Carabaño, M.J. “Validación de genes de referencia normalizadotes de la expresión génica para la respuesta a estrés térmico en la especie ovina”. En: 41 Jornadas de Estudio. XIV Jornadas sobre Producción Animal. AIDA. Zaragoza, España, 17 y 18 de mayo de 2011, pp.497-499. ISBN: 978-84-615-0062-8 |
| Abstract: | We have tested 16 putative reference genes in peripheral whole blood of control and heat stressed ovine samples to detect stable genes for the heat-shock response. To determine gene expression stability a new approach based in Maximum Likelihood estimation of Mixed Model parameters has been tested and compared to commonly used software (NormFinder and geNorm). Differences in the stability rankings of genes were found among the three approaches. Under our approach SDHA and MDH1 was the best set of genes to be normalizers of target genes in heat shock experiments in the ovine specie |
| URI: | http://hdl.handle.net/10532/2021 |
| Appears in Collections: | [DOCIART] Artículos científicos, técnicos y divulgativos |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 2011_176.pdf | 74,42 kB | Adobe PDF |  View/Open |
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