In vitro Micro-Vibration Increases Implantation Rate after Embryonic Cell Transplantation

Abstract

In natural conditions the oocyte and embryo are subjected to ever changing dynamic processes. However, the routine assisted reproductive technologies today involve the use of static in vitro culture systems. Objective was to determine whether there is any difference in the viability of embryos after in vitro culture under static and mechanical micro-vibration condition. It was evaluated the viability of embryonic cells (9,624 embryos) generated from 4,436 couples after in vitro culture. For Groups <29 years, 30-34 years, 35-39 years and >40 years, the following rate of high quality embryos without fragmentation (2 to 4 blastomeres on Day 2; 6 to 8 blastomeres and compacting morula on Day 3; blastocyst, expanded and hatching blastocyst on Day 5) was detected (static vs. vibration, respectively): 65% vs. 71%, 44% vs. 69%, 67% vs. 76% (for statistic significant differences between respective rates in these three groups P<0.05) and 67% vs. 66% (P>0.1). The following baby-take-home rate was detected for Groups <29 years, 30-34 years, 35-39 years and >40 years, (static vs. vibration, respectively): 30% vs. 31% (P>0.1, increasing only on the level of tendency), 28% vs. 37%, 23% vs. 29% and 9% vs. 15% (differences between respective rates in these three groups with P<0.05). It was concluded that in vitro culture of embryos under micro-vibration (with a mimic of conditions in nature whereby oviductal fluid is mechanically agitated by the epithelial cilia) significantly increases baby-take-home rate for patients of 30 years and older.