Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10532/7139
Título : Genome editing in almond: A CRISPR-based approach through hairy root transformation
Autor : Jedličková, Veronika
Štefková, Marie
Sánchez López, Juan Francisco
Grimplet, Jérôme
Rubio Cabetas, María José
Robert, Hélène S.
Fecha de publicación : 2024
Citación : Biorxiv, 2024
Resumen : Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond.
URI : http://hdl.handle.net/10532/7139
Documento relativo: https://doi.org/10.1101/2024.04.11.588989
Licencia: http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Aparece en las colecciones: [DOCIART] Artículos científicos, técnicos y divulgativos

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