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Title: Genome editing in almond: A CRISPR-based approach through hairy root transformation
Authors: Jedličková, Veronika
Štefková, Marie
Sánchez López, Juan Francisco
Grimplet, Jerome
Rubio Cabetas, María José
Robert, Hélène S.
Issue Date: 2024
Citation: Biorxiv, 2024
Abstract: Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond.
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Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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