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Campo DC | Valor | Idioma |
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dc.contributor.author | Jedličková, Veronika | es_ES |
dc.contributor.author | Štefková, Marie | es_ES |
dc.contributor.author | Sánchez López, Juan Francisco | es_ES |
dc.contributor.author | Grimplet, Jérôme | es_ES |
dc.contributor.author | Rubio Cabetas, María José | es_ES |
dc.contributor.author | Robert, Hélène S. | es_ES |
dc.coverage.spatial | Ciencia vegetal | es_ES |
dc.date.accessioned | 2024-07-05T11:58:43Z | - |
dc.date.available | 2024-07-05T11:58:43Z | - |
dc.date.issued | 2024 | es_ES |
dc.identifier.citation | Biorxiv, 2024 | - |
dc.identifier.uri | http://hdl.handle.net/10532/7139 | - |
dc.description.abstract | Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond. | en |
dc.description.sponsorship | This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic within the program INTER-COST (LTC20004) and from the project TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius. | es_ES |
dc.language.iso | en | es_ES |
dc.relation.uri | https://doi.org/10.1101/2024.04.11.588989 | es_ES |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | es |
dc.title | Genome editing in almond: A CRISPR-based approach through hairy root transformation | en |
dc.type | Journal Contribution | * |
dc.date.updated | 2024-06-19T17:06:01Z | - |
dc.subject.agrovoc | Prunus dulcis | es |
dc.subject.agrovoc | Genomas | es |
dc.subject.agrovoc | Genes | es |
dc.description.other | Prunus dulcis | en |
dc.description.other | hairy root culture | en |
dc.description.other | composite plants | en |
dc.description.other | CRISPR/Cas9 | en |
dc.description.other | ERF74 | en |
dc.description.other | GAI | en |
dc.description.status | Published | es_ES |
dc.type.refereed | Refereed | es_ES |
dc.type.specified | Article | es_ES |
dc.bibliographicCitation.title | BioRxiv | en |
dc.relation.doi | https://doi.org/10.1101/2024.04.11.588989 | es_ES |
dc.relation.data | https://www.biorxiv.org/content/10.1101/2024.04.11.588989v1.full | es_ES |
Aparece en las colecciones: | [DOCIART] Artículos científicos, técnicos y divulgativos |
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