Please use this identifier to cite or link to this item: http://hdl.handle.net/10532/7139
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dc.contributor.authorJedličková, Veronikaes_ES
dc.contributor.authorŠtefková, Mariees_ES
dc.contributor.authorSánchez López, Juan Franciscoes_ES
dc.contributor.authorGrimplet, Jeromees_ES
dc.contributor.authorRubio Cabetas, María Josées_ES
dc.contributor.authorRobert, Hélène S.es_ES
dc.coverage.spatialCiencia vegetales_ES
dc.date.accessioned2024-07-05T11:58:43Z-
dc.date.available2024-07-05T11:58:43Z-
dc.date.issued2024es_ES
dc.identifier.citationBiorxiv, 2024-
dc.identifier.urihttp://hdl.handle.net/10532/7139-
dc.description.abstractClustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond.en
dc.description.sponsorshipThis work was supported by the Ministry of Education, Youth and Sports of the Czech Republic within the program INTER-COST (LTC20004) and from the project TowArds Next GENeration Crops, reg. no. CZ.02.01.01/00/22_008/0004581 of the ERDF Programme Johannes Amos Comenius.es_ES
dc.language.isoenes_ES
dc.relation.urihttps://doi.org/10.1101/2024.04.11.588989es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es
dc.titleGenome editing in almond: A CRISPR-based approach through hairy root transformationen
dc.typeJournal Contribution*
dc.date.updated2024-06-19T17:06:01Z-
dc.subject.agrovocPrunus dulcises
dc.subject.agrovocGenomases
dc.subject.agrovocGeneses
dc.description.otherPrunus dulcisen
dc.description.otherhairy root cultureen
dc.description.othercomposite plantsen
dc.description.otherCRISPR/Cas9en
dc.description.otherERF74en
dc.description.otherGAIen
dc.description.statusPublishedes_ES
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES
dc.bibliographicCitation.titleBioRxiven
dc.relation.doihttps://doi.org/10.1101/2024.04.11.588989es_ES
dc.relation.datahttps://www.biorxiv.org/content/10.1101/2024.04.11.588989v1.fulles_ES
Appears in Collections:[DOCIART] Artículos científicos, técnicos y divulgativos

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