New methodology for genomic characterization of S-alleles in Japanese plum cultivars

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dc.contributor.authorTorres Punina, Andrea de las Mercedes
dc.contributor.authorGuerra Velo, Mª Engracia
dc.contributor.authorRodrigo García, Francisco Javier
dc.contributor.authorHedhly, Afif
dc.contributor.orcidRodrigo García, Francisco Javier [0000-0002-8321-1764]
dc.date.accessioned2026-01-28T11:45:21Z
dc.date.available2026-01-28T11:45:21Z
dc.date.issued2026-01-14
dc.date.updated2026-01-27T11:34:00Z
dc.description.abstractPrunus species have a gametophytic incompatibility system that prevents self-fertilization and promotes crosspollination. This system is regulated by the multiallelic S locus, formed by two closely linked genes, the S-RNase expressed in the pistil and the SFB in the pollen tube. The identification of both S alleles of a cultivar is essential to establish its pollination needs, as well as its suitability as a parent in breeding programs. To date, in Japanese plum (hybrids of Prunus salicina Lindl.), 57 S alleles have been described (42 S-RNase and 15 SFB), which has allowed the assignment of more than 200 cultivars to 26 incompatibility groups. However, identification of S alleles has not been possible in a number of cultivars, presumably because their S alleles lack length polymorphism with already known alleles and have not been previously sequenced. In this work, we resequenced the genome of nine Japanese plum cultivars and applied a novel NGS-based methodology for S-genotyping and targeted de novo assembly of S-alleles and loci.
dc.description.sponsorshipEsta investigación ha sido financiada por el proyecto PID2020-115473RR-I00, financiado por el MICIU/AEI/10.13039/501100011033, y el Gobierno de Aragón-Fondo Social Europeo, Unión Europea (Grupo Consolidado A12_23R).
dc.identifier.citationTorres, A., Guerra, M. E., Rodrigo, J., & Hedhly, A. (2026). New methodology for genomic characterization of S-alleles in Japanese plum cultivars. En: Jornada Investigadores Predoctorales IA2 - Campus Aula Dei 2026 (Zaragoza. España. 2026)
dc.identifier.urihttps://hdl.handle.net/10532/8117
dc.language.isoen
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115473RR-I00/ES/REPOSO Y DESARROLLO DE LA FLOR EN FRUTALES DE CLIMA TEMPLADO/REPFRUT
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 4.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/es/
dc.subject.agrovocPrunus salicina
dc.subject.agrovocAlelo
dc.subject.agrovocIncompatibilidad del polen
dc.subject.agrovocGenómica
dc.subject.sdgHambre cero
dc.titleNew methodology for genomic characterization of S-alleles in Japanese plum cultivars
dc.typetexto
dc.typecontribución de congreso
dc.typepóster no publicado en actas de congreso
dc.type.hasVersionversión publicada

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