In vitro early detection of grapevine virus-induced graft incompatibility in Syrah/R110: the involvement of GRSPaV
Loading...
Date
2023
Authors
Tedesco, SaraIrisarri Sarto, Patricia
Teixeira-Santos, Margarida
Fevereiro, Pedro
Pina Sobrino, Ana
Kragler, Friedrich
Journal Title
Journal ISSN
Volume Title
Publisher
Typology
conferenceObjectPoster
Abstract
In grapevines, incompatibility manifests in short-term graft failure or long-term decline of vineyards both causing economic losses to nurseries and growers. Here, we assessed the suitability of in vitro systems coupled with histochemistry analysis as early detection methods for grapevine incompatibility using certified homografted (i.e., a graft between the same genotype) and heterografted (i.e., a graft between two genotypes) grapevine micrografts, with known graft compatibility response when grafted onto the worldwide used rootstock 110R (V. berlandieri x V. rupestris). To prove the involvement of Grapevine rupestris stem pitting-associated virus (GRSPaV) in Syrah/110R incompatibility, we silenced GRSPaV transcripts by ectopically applying a GRSPaV siRNA-inducing dsRNA.
Description
Keywords
Bibliographic citation
Tedesco, S., Irisarri, P., Teixeira-Santos, M., Fevereiro, P., Pina, A. & Kragler, F. (2023). In vitro early detection of grapevine virus-induced graft incompatibility in Syrah/R110: the involvement of GRSPaV. 20th Conference of the International Council for the Study of Virus and Virus-Like Diseases of the Grapevine (Thessaloniki. Grecia. 2023).
AGROVOC subjects
VidIncompatibilidad del injerto
Cultivo in vitro
Portainjertos
Betaflexiviridae
Enfermedades de las plantas