Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility.

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dc.bibliographicCitation.titleAnimal: The international journal of animal biosciencesen
dc.bibliographicCitation.volume19es_ES
dc.contributor.authorToquet, M.es_ES
dc.contributor.authorGomis, J.es_ES
dc.contributor.authorJiménez-Trigos, E.es_ES
dc.contributor.authorBataller, E.es_ES
dc.contributor.authorBarba Recreo, Marta es_ES
dc.contributor.authorSánchez, A.es_ES
dc.contributor.authorGonzález-Torres, P.es_ES
dc.contributor.authorGómez-Martín, A.es_ES
dc.coverage.spatialcitaTEes_ES
dc.date.accessioned2025-03-26T11:43:07Z
dc.date.available2025-03-26T11:43:07Z
dc.date.issued2025es_ES
dc.date.updated2025-03-11T13:33:14Z
dc.description.abstractIn vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains of Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and L. gasseri) against some ruminants' pathogens. However, their possible beneficial effects on controlling the vaginosis caused by the use of intravaginal sponges for oestrus synchronisation have not been evaluated in in vivo models. Therefore, the purpose of this study was to perform an intravaginal double inoculation of this same non-lyophilised (non-freeze-dried) L2 inoculum, in 120 ewes from four dairy commercial ovine flocks using straws similar to those used in artificial insemination. Globally, in the entire study population, the fertility percentage (65%) of the inoculated groups increased by 10% compared to the control groups (55%). Specifically, increases in the percentages of pregnant sheep in flocks 1 and 4 (10.7 and 5.7% respectively; P > 0.05) and flock 3 (27.3%; P < 0.05) were observed in inoculated ewes. Overall, the administration of L2 on the day of intravaginal sponge insertion decreased the number of ewes with vaginitis by 11.13% at the time of sponge removal (T1). In the first flock, the administration of the probiotic positively modulated the microbiota with an increased richness and presence of Lactobacillales accompanied by a reduction in Mycoplasma spp. Genera such as Fusobacterium, Streptobacillus, Campylobacter or Trueperella were linked to the presence of vaginitis at T1. Genera belonging to Lactobacillales, including some genera previously reported to be beneficial for pregnancy, significantly increased at the time of pregnancy diagnosis but significantly decreased at T1. The results of this study suggest that the use of probiotics via intravaginal inoculation as a strategy to positively modulate the microbiota and vaginal inflammation, derived from the use of intravaginal sponges, is also associated with improved fertility. This novel approach could improve animal welfare in oestrus-synchronisation protocols and represents an alternative to the prophylactic use of antibiotics.en
dc.description.otherAlternative to antibiotic therapyen
dc.description.otherAnimalen
dc.description.otherwelfareen
dc.description.otherEwesen
dc.description.otherLactobacillusen
dc.description.otherMollicutesen
dc.description.sponsorshipWe would like to thank the livestock associations AGRAMA, ASSAFE and CONFELAC, but also Neiker (Basque Institute for Agricultural Research and Development) and OVIGEN (centre for selection and genetic improvement of sheep and goats of Castilla y León) for their participation in this study.es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationToquet, M.; Gomis, J.; Jiménez-Trigos, E.; Bataller, E.; Barba, M.; Sánchez, A.; González-Torres, P.; Gómez-Martín, A. Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility. Animal, 2025, 19, 2, 101417
dc.identifier.issn17517311
dc.identifier.urihttp://hdl.handle.net/10532/7551
dc.language.isoenes_ES
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dc.relation.doihttps://doi.org/10.1016/j.animal.2024.101417es_ES
dc.relation.urihttps://doi.org/10.1016/j.animal.2024.101417es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocResistencia a los antibióticoses
dc.subject.agrovocBienestar animales
dc.subject.agrovocLactobacilluses
dc.subject.agrovocOvinoses
dc.subject.agrovocOvejaes
dc.subject.agrovocProbióticoses
dc.subject.otherAlternative to antibiotic therapy
dc.subject.otherAnimal Welfare
dc.subject.otherBienestar animal
dc.subject.otherEWES
dc.subject.otherLactobacillus
dc.subject.otherMollicutes
dc.subject.otherOveja
dc.subject.otherOvinos
dc.subject.otherResistencia A Los Antibióticos
dc.titleDevelopment of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility.en
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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