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Título : Detection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genome
Autor : Bielsa Pérez, Beatriz
Jiwan, D
Fernández i Martí, Angel V.
Dhingra, Amit
Rubio Cabetas, María José
Fecha de publicación : 2014
Citación : Bielsa, B; Jiwan, D.; Fernandez i Marti, A.; Dhingra, A.; Rubio-Cabetas, M. J. Detection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genome. Scientia Horticulturae, 2014, 168, 108-112
Resumen : In order to control tree size, disease, precocity and stress most Prunus varieties are cultivated as composite plants grafted onto desirable rootstocks that impart all the afore-mentioned traits. Several Prunus rootstock breeding programs have been focused on the production of interspecific hybrids. The pedigree of most of these rootstocks remains unknown due to the lack of parental information necessitating the application of DNA-based knowledge in breeding programs. The amplification and sequencing of the chloroplast inverted repeat B (IRB) region spanning 25,960. bp from P. cerasifera (myrobalan plum) Ehrh., P. amygdalus (almond) and P. persica (peach) using the ASAP method revealed a single nucleotide polymorphisms (SNP) in the rps19. rpl2 IRB region in myrobalan when compared to almond and peach. In addition, a prominent and an easily identifiable single feature polymorphism (SFP-InDel (deletion)) of 18 nucleotides was discovered in reference to the peach chloroplast genome in the ycf1 gene in the IRB region. In this work, it has been developed a highly useful polymorphic molecular marker to characterize the maternal parent in interspecific hybrids of Prunus rootstocks as a first step toward developing pedigree information. The ycf1 SFP-InDel (deletion) has been successfully used in several 3-way hybrids generated in the stone fruit rootstock breeding program for the characterization of new interspecific plant material. This SFP is expected to be highly utile in characterizing the maternal lineage of Prunus hybrids in other breeding programs. © 2014.
URI : http://hdl.handle.net/10532/7462
Documento relativo: https://doi.org/10.1016/j.scienta.2014.01.028
ISSN : 0304-4238
Licencia: http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Aparece en las colecciones: [DOCIART] Artículos científicos, técnicos y divulgativos

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