Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation

Simple item page
dc.contributor.authorBosch, Camila
dc.contributor.authorGarcía López, Carla
dc.contributor.authorSaralegui Remón, Luis
dc.contributor.authorvan Beek, Lucille
dc.contributor.authorde Jonge, Marien I.
dc.contributor.authorMarín Alcalá, Clara María
dc.contributor.funderAgencia Estatal de Investigación
dc.contributor.orcidMarín Alcalá, Clara María [0000-0002-1974-9025]
dc.date.accessioned2025-05-14T12:02:13Z
dc.date.available2025-05-14T12:02:13Z
dc.date.issued2025-05-08
dc.description.abstractStreptococcus suis is a Gram-positive bacterium responsible for various infections in both pigs and humans. This study investigates the role of methionine acquisition in the growth and virulence of S. suis. The putative methionine transport system is organised as an operon comprising the metQ gene and genes encoding a transposase and an ATPase, forming a typical tripartite ABC transporter. This operon is conserved across multiple streptococcal species, including both animal and human pathogens. We examined whether MetQ functions as a methionine-binding protein and its role in bacterial infection. Using Western blotting and flow cytometry with a specific antiserum, we demonstrated that MetQ is produced in vitro by the S. suis reference strain P1/7 under methionine-limited conditions and is located on the bacterial cell surface. Growth assays in chemically defined media revealed that a metQ deletion mutant (P1/7∆metQ) exhibited impaired growth under methionine-restricted conditions but grew normally in a nutrient-rich medium, suggesting that MetQ primarily transports methionine. Isothermal Titration Calorimetry demonstrated that MetQ binds L-methionine with a dissociation constant (KD) of 7.1 µM. In a murine infection model, the metQ mutant showed reduced dissemination to internal organs compared to the wild type. Furthermore, the mutant showed decreased intracellular survival in murine macrophages and increased sensitivity to oxidative stress, while exhibited enhanced biofilm formation compared to the wild type. Our findings indicate that MetQ is essential for methionine uptake under methionine-restricted conditions, which is critical for bacterial nutrition, immune evasion, and pathogenicity during infection.
dc.description.peerreviewedSi
dc.description.sponsorshipEste trabajo recibió financiación del Ministerio de Ciencia e Innovación/Agencia Española de Investigación (MCIN/AEI) y, según corresponda, del Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa” por la Unión Europea o por la Unión Europea NextGenerationEU/PRTR (Acuerdo de subvención PID2020-114617RB-100). Los financiadores no tuvieron ningún papel en el diseño del estudio, la recogida y el análisis de datos, las decisiones de publicación ni en la red
dc.formatapplication/pdf
dc.identifier.citationBosch, C., García, C., Saralegui, L., Van Beek, L., De Jonge, M. I., Marín, C., & Arenas, J. (2025). Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation. Veterinary Research, 56(1), 99. https://doi.org/10.1186/s13567-025-01522-y
dc.identifier.issn1297-9716
dc.identifier.urihttps://hdl.handle.net/10532/7605
dc.language.isoen
dc.publisherSpringer-Nature
dc.relationPID2020-114617RB-100
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.agrovocStreptococcus suis
dc.subject.agrovocMetionina
dc.subject.agrovocFagocitosis
dc.subject.agrovocEstrés oxidativo
dc.subject.sdgSalud y bienestar
dc.subject.sdgHambre cero
dc.subject.sdgProducción y consumo responsables
dc.subject.sdgVida de ecosistemas terrestres
dc.titleIdentification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión publicada

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
10178666.pdf
Size:
3.51 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos científicos, técnicos y divulgativos