Unravelling molecular mechanisms involved in resistance priming against downy mildew (Plasmopara viticola) in grapevine (Vitis vinifera L.)
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Date
2023
Authors
Vigneron, NicolasGrimplet, Jérôme
Remolif, Eric
Rienth, Markus
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Abstract
Downy mildew (DM; Plasmopara viticola) is amongst the most severe fungal diseases in viticulture and the reason for the majority of fungicide applications. To reduce synthetic and copper-based fungicides, there is an urgent need for natural alternatives, which are being increasingly tested by the industry and the research community. However, their mode of action remains unclear. Therefore, our study aimed to investigate the transcriptomic changes induced by oregano essential oil vapour (OEOV) in DM-infected grapevines. OEOV was applied at different time points before and after DM infection to differentiate between a priming effect and a direct effect. Both pre-DM treatment with OEOV and post-infection treatment resulted in a significant reduction in DM sporulation. RNA-seq, followed by differential gene expression and weighted gene co-expression network analysis, identified co-expressed gene modules associated with secondary metabolism, pathogen recognition and response. Surprisingly, the molecular mechanisms underlying the efficiency of OEOV against DM appear to be independent of stilbene synthesis, and instead involve genes from a putative signalling pathway that has yet to be characterized. This study enhances our understanding of the molecular regulation of innate plant immunity and provides new insights into the mode of action of alternative natural antifungal agents.
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Vigneron, N., Grimplet, J., Remolif, E., & Rienth, M. (2023). Unravelling molecular mechanisms involved in resistance priming against downy mildew (Plasmopara viticola) in grapevine (Vitis vinifera L.). Scientific Reports, 13(1), Article 14664.
AGROVOC subjects
MildiuBioplaguicidas
Transcriptomas
Orégano
Inmunidad natural
Sponsorship
Deseamos expresar nuestro agradecimiento a la Fundación Nacional Suiza para la Ciencia (SNSF) por la financiación del proyecto en el marco del cual se llevó a cabo el estudio (subvención número IZCOZ0_189896). También nos gustaría dar las gracias a la acción de costes INTEGRAPE (CA17111) por brindar la oportunidad de presentar la propuesta de proyecto y por todo el intercambio con colegas que fue posible a través de esta acción COST.