Multilocus Variable Number of Tandem Repeat Analysis Reveals Multiple Introductions in Spain of Xanthomonas arboricola pv. pruni, the Causal Agent of Bacterial Spot Disease of Stone Fruits and Almond
Loading...
Date
2016
Authors
López Soriano, P.Boyer, Karine
Cesbron, Sophie
Morente, M.C.
Peñalver, J.
Palacio Bielsa, Ana
Vernière, Christian
López, M.M.
Pruvost, Olivier
Journal Title
Journal ISSN
Volume Title
Publisher
Typology
Journal ContributionArticle
Abstract
Xanthomonas arboricola pv. pruni is the causal agent of the bacterial spot disease of stone
fruits, almond and some ornamental Prunus species. In Spain it was first detected in 2002
and since then, several outbreaks have occurred in different regions affecting mainly Japanese
plum, peach and almond, both in commercial orchards and nurseries. As the origin of
the introduction(s) was unknown, we have assessed the genetic diversity of 239 X. arboricola
pv. pruni strains collected from 11 Spanish provinces from 2002 to 2013 and 25 reference
strains from international collections. We have developed an optimized multilocus
variable number of tandem repeat analysis (MLVA) scheme targeting 18 microsatellites
and five minisatellites. A high discriminatory power was achieved since almost 50% of the
Spanish strains were distinguishable, confirming the usefulness of this genotyping technique
at small spatio-temporal scales. Spanish strains grouped in 18 genetic clusters (conservatively
delineated so that each cluster contained haplotype networks linked by up to
quadruple-locus variations). Furthermore, pairwise comparisons among populations from
different provinces showed a strong genetic differentiation. Our results suggest multiple
introductions of this pathogen in Spain and redistribution through contaminated nursery
propagative plant material.
Description
Keywords
Bibliographic citation
Plos One (Septiembre), p. 1-18
AGROVOC subjects
XanthomonasFitopatología
Árboles frutales
Enfermedades de las plantas
Other field subjects
sanidad vegetalSponsorship
This work was supported by Instituto
Nacional de Tecnología Agraria y Alimentaria.
Project RTA-2011-00140-C03-01 (http://www.inia.
es) to PLS MCM JP MML, Euphresco project
(266505-FP7-ERANET Euphresco II) to PLS MCM
JP APB MML, European Social Fund to PLS KB
MCM JP APB CV MML OP, European Regional