Characterization of bacterial communities of ewe’s vaginal tract and its potential impact on reproductive efficiency

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dc.contributor.authorReinoso Peláez, Edgar L.
dc.contributor.authorPuente Sánchez, F.
dc.contributor.authorSerrano Noreña, Magdalena
dc.contributor.authorCalvo Lacosta, Jorge Hugo
dc.contributor.authorRamón, Manuel
dc.contributor.authorSaura, María
dc.contributor.funderAgencia Estatal de Investigación
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades
dc.contributor.funderSwedish Research Council
dc.contributor.orcidCalvo Lacosta, Jorge Hugo [0000-0001-9513-0219]
dc.date.accessioned2025-05-20T10:09:40Z
dc.date.available2025-05-20T10:09:40Z
dc.date.issued2025-05-14
dc.description.abstractThe success rate of artificial insemination in sheep remains suboptimal, which has led to an emerging interest in the impact of the reproductive tract microbiome on this process. This research aims to identify the ewes’ vaginal core bacterial community, examine the factors influencing bacterial composition, and to determine the association between vaginal bacteria and pregnancy success. By using a robust dataset comprising 331 multiparous ewes from three Spanish breeds (Latxa, Manchega, Rasa Aragonesa) across four herds, this study performed the sequencing of the hypervariable regions V3-V4 of the 16S ribosomal RNA gene and the identification of Amplicon Sequence Variants (ASV) to analyze the bacterial community. Our analysis revealed a core bacterial primarily consisting of the genera Streptobacillus, Histophilus, Fusobacterium, Oceanivirga, and Parvimonas. Alpha and beta diversity, as well as Random Forest analysis, identified that herd and breed were the main drivers of bacterial variability. PERMANOVA analysis also showed significant differences in bacterial composition and abundance associated with pregnancy outcomes. Notably, specific ASVs associated with Fusobacterium, Leptotrichia, Histophilus, Escherichia, and Bacteroides were predominantly found in non-pregnant ewes, while genera such as Pseudomonas, Acinetobacter, and Brevundimonas were more abundant in pregnant ewes. This study contributes to the knowledge about the critical roles of specific bacteria in determining reproductive success in sheep and provides novel insights about the importance of different factors involved in the composition of ewes’ vaginal bacterial communities.
dc.description.peerreviewedSi
dc.description.sponsorshipEste trabajo fue financiado por la subvención RTI-2018-096487-R-C33 del Ministerio de Ciencia, Innovación y Universidades, España; CSIC (2022 AEP004), España; y Fondos FEDER. ELR-P está apoyado por una beca FPI PRE2019-090087 del Ministerio de Ciencia, Innovación y Universidades. FP-S está apoyado por la subvención 2022-04801 del Consejo de Investigación de Suecia
dc.identifier.citationReinoso-Peláez, E. L., Puente-Sánchez, F., Serrano, M., Calvo, J. H., Ramón, M., & Saura, M. (2025). Characterization of bacterial communities of ewe’s vaginal tract and its potential impact on reproductive efficiency. Animal Microbiome, 7(1), 48. https://doi.org/10.1186/s42523-025-00383-2
dc.identifier.doi10.1186/s42523-025-00383-2
dc.identifier.issn2524-4671
dc.identifier.urihttps://hdl.handle.net/10532/7610
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.relationinfo:eu-repo/grantAgreement/AEI/Retos Investigación: Proyectos I+D+i/RTI2018-096487-R-C33
dc.relation.citaSi
dc.relation.ispartofAnimal Microbiome
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectArtificial insemination
dc.subjectBacterial communities
dc.subjectFertility
dc.subjectOvine
dc.subjectReproductive success
dc.subjectVaginal microbiota
dc.subject.agrovocAi (artificial insemination)
dc.subject.agrovocMicrobiota
dc.subject.agrovocFertilidad
dc.subject.agrovocOvino
dc.subject.agrovocTasa de partos en ganado ovino
dc.subject.sdgHambre cero
dc.subject.sdgIndustria, innovación e infraestructura
dc.subject.sdgProducción y consumo responsables
dc.titleCharacterization of bacterial communities of ewe’s vaginal tract and its potential impact on reproductive efficiency
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión publicada
oaire.citation.issue1
oaire.citation.volume7

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