Effect of crude protein level in lamb feed on circulating malondialdehyde and cytokine expression in rumen and ileum

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dc.bibliographicCitation.conferencedate26, 27 y 28 de septiembre de 2019es_ES
dc.bibliographicCitation.conferencenameCongreso Internacional de la Federación Mediterránea de Sanidad y Producción de Rumiantes (FeMeSPRum)es
dc.bibliographicCitation.conferenceplaceLeónes_ES
dc.contributor.authorPelegrín Valls, Jonathanes_ES
dc.contributor.authorMartín Alonso, María Josées_ES
dc.contributor.authorBertolín Pardos, Juan Ramónes_ES
dc.contributor.authorPérez, M.es_ES
dc.contributor.authorVillalba Mata, Danieles_ES
dc.contributor.authorAlvarez Rodríguez, Javieres_ES
dc.contributor.authorSerrano Pérez, Beatrizes_ES
dc.coverage.spatialProducción y sanidad animales_ES
dc.date.accessioned2021-11-04T11:53:07Z
dc.date.available2021-11-04T11:53:07Z
dc.date.issued2019es_ES
dc.description.abstractiets for intensive light lambs in Spain have crude protein (CP) levels above their nutritional requirements. To optimize feed costs, it is necessary to understand how CP reduction affects the balance between immune mechanisms of defence or tolerance in the gastrointestinal tract. Low protein diets have been linked to oxidative stress (OS) status, which is associated with the formation of free radicals and pro-inflammatory cytokines, causing damage in the intestinal mucosa. In addition, as a product of lipid peroxidation, an animal with OS develops high levels of malondialdehyde (MDA), which can be evaluated in blood plasma as an indicator of oxidative status. The aim of this study was to assess the effect of dietary CP on circulating MDA concentration and gene expression of pro-inflammatory (TNF-alpha) and anti-inflammatory (IL-10 and TGF-beta) cytokines as indicators of ruminal and intestinal health. Ripollesa male lambs aged 45–60 days were used (n = 120). The animals were fed in two phases: growth from 14 to 19 kg and fattening from 19 to 25 kg of body weight. Half of the lambs received a control CP in feed (20.4% and 19.1% on a dry matter basis (DM), respectively) and the rest received a low CP content (18.3% and 17.4% on DM). Plasma MDA concentrations were determined in blood samples taken from 48 lambs (n = 24 for each treatment) in the last week of each phase by liquid chromatography with diode detector (HPLC-DAD). At the time of slaughter, rumen and ileum samples were taken from 8 lambs (n = 4 for each treatment), which were kept in RNA later until extraction. RNA was extracted according to the Trizol protocol (Invitrogen), treated with DNases, and DNA copy was obtained by reverse transcription in the presence of random primers. Gene expression of TNF-alpha, IL-10, TGF-beta, and reference gene GAPDH was determined by real-time PCR in ruminal and ileum samples. The results showed that MDA concentrations were not different between control and low CP treatments, neither in the growth phase (7.30 vs 7.41 ± 0.21 μM respectively, P>0.05) nor in the fattening phase (7.06 vs 7.43 ± 0.32 μM respectively, P>0.05). There were no significant differences in cytokine gene expression in rumen and ileum at slaughter between control animals and those fed low CP (P>0.05). In conclusion, the reduction of CP in lambs’ diet did not negatively affect their oxidative blood status nor did it alter the immune response in the gastrointestinal tract.en
dc.identifier.citationCongreso Internacional de la Federación Mediterránea de Sanidad y Producción de Rumiantes (FeMeSPRum). León, 26, 27 y 28 de septiembre de 2019
dc.identifier.urihttp://hdl.handle.net/10532/5606
dc.language.isoenes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.agrovocOvinoses
dc.subject.agrovocCorderoes
dc.subject.agrovocNutrición animales
dc.titleEffect of crude protein level in lamb feed on circulating malondialdehyde and cytokine expression in rumen and ileumen
dc.title.alternativeEfecto del nivel de proteína bruta en el pienso de cebo de corderos sobre el malondialdehido plasmático y la expresión de citoquinas en rumen e íleones
dc.typeConference Material*
dc.type.refereedRefereedes_ES
dc.type.specifiedPosteres_ES

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