Melatonin supplementation does not improve ovine pre- or post-hatching development in vitro

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dc.contributor.authorCarvajal Serna, Melissa
dc.contributor.authorMartínez de los Reyes, Nuria
dc.contributor.authorMarigorta del Val, P.
dc.contributor.authorBermejo-Álvarez, Pablo
dc.contributor.authorRamos Ibeas, Priscila
dc.contributor.funderAgencia Estatal de Investigación
dc.contributor.funderMinisterio de Ciencia e Innovación
dc.contributor.orcidCarvajal Serna, Melissa [0000-0003-3929-5064]
dc.date.accessioned2025-07-01T12:09:35Z
dc.date.available2025-07-01T12:09:35Z
dc.date.issued2025-04-09
dc.date.updated2025-06-20T05:45:32Z
dc.description.abstractMelatonin plays a crucial role in the regulation of reproductive seasonality in sheep. Melatonin supplementation has been widely used both in vivo to increase fertility and prolificacy, and in vitro during oocyte maturation, fertilization, and embryo culture. However, its effects have only been assessed in vitro up to the hatched blastocyst stage in conventional embryo culture systems. This study aimed to evaluate the effects of melatonin supplementation from oocyte in vitro maturation (IVM) through post-hatching embryonic stages in vitro on the development of the first three embryonic lineages. Supplementation with melatonin at 10−8 or 10−6 M during IVM, in vitro fertilization (IVF), and in vitro culture (IVC) did not affect cleavage and blastocyst rates at day (D) 8, nor embryo survival and growth at D12. While hypoblast development was not affected, epiblast survival was reduced in D12 embryos treated with 10−6 M melatonin from the blastocyst stage onward. In conclusion, melatonin supplementation did not show a clear beneficial effect on ovine in vitro embryo production or lineage development during post-hatching embryo culture.
dc.description.peerreviewedSi
dc.description.sponsorshipEste trabajo ha sido financiado por los proyectos PID2021-122153NA-I00 del Ministerio de Ciencia e Innovación de España a PRI, y ECQ2018-005184-P del Ministerio de Ciencia e Innovación de España a PBA. Melissa Carvajal fue financiado por una beca Margarita Salas.
dc.identifier.citationCarvajal-Serna, M., Martínez de los Reyes, N., Marigorta, P., Bermejo-Álvarez, P., & Ramos-Ibeas, P. (2025). Melatonin supplementation does not improve ovine pre- or post-hatching development in vitro. Theriogenology, 241, 117428. https://doi.org/10.1016/j.theriogenology.2025.117428
dc.identifier.doi10.1016/j.theriogenology.2025.117428
dc.identifier.issn0093-691X
dc.identifier.urihttps://hdl.handle.net/10532/7666
dc.language.isoen
dc.publisherElsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122153NA-I00
dc.relationinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/ECQ2018-005184-P
dc.relation.citaSi
dc.relation.publisherversionhttps://doi.org/10.1016/j.theriogenology.2025.117428
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.agrovocMelatonina
dc.subject.agrovocEstacionalidad
dc.subject.agrovocOvino
dc.subject.agrovocCultivo in vitro
dc.subject.agrovocDesarrollo embrionario
dc.subject.sdgSalud y bienestar
dc.subject.sdgProducción y consumo responsables
dc.subject.sdgVida de ecosistemas terrestres
dc.titleMelatonin supplementation does not improve ovine pre- or post-hatching development in vitro
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión publicada

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