S-genotyping in Japanese plum by PCR and capillary gel electrophoresis detection

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dc.bibliographicCitation.endpage142es_ES
dc.bibliographicCitation.stpage139es_ES
dc.bibliographicCitation.titleActa Horticulturaeen
dc.bibliographicCitation.volume932es_ES
dc.contributor.authorGuerra Velo, Mª Engraciaes_ES
dc.contributor.authorLópez Corrales, Margaritaes_ES
dc.contributor.authorRodrigo García, Francisco Javieres_ES
dc.contributor.authorWünsch, Anaes_ES
dc.date.accessioned2013-02-14T09:34:35Z
dc.date.available2013-02-14T09:34:35Z
dc.date.issued2012es_ES
dc.description.abstractIn this work a PCR S-genotyping method using capillary electrophoresis detection was assayed in Japanese plum. Sweet cherry primers designed for S-RNase and SFB intron length polymorphism detection by capillary electrophoresis were evaluated in Japanese plum cultivars. Amplification of both genes was successful and amplified sizes were correlated with Japanese plum S-alleles. The S-RNase genotype of 58 Japanese plum type cultivars previously determined by other methods was confirmed using this technology and the SFB alleles of these cultivars were also determined. Allele sizes of both genes are reported for 13 different S-alleles found in Japanese plum and will allow efficient S-genotype characterization in this species.en
dc.description.othergamenophytic self-incompatibilityen
dc.description.otherS-RNaseen
dc.description.otherSFBen
dc.description.otherS-alleleen
dc.description.otherPrunus salicina Lindlen
dc.description.statusPublishedes_ES
dc.description.versionPeer Reviewed
dc.identifier.citationGuerra , M.E., López-Corrales, M., Rodrigo , J. and Wünsch, A. (2012). “S-genotyping in Japanese plum by PCR and capillary gel electrophoresis detection” XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Plant Physiology from Cell to Fruit Production System. Lisbon, Portugal, August 22-27, 2010. Acta Hort. (ISHS) 932:139-142
dc.identifier.issn0567-7572*
dc.identifier.urihttp://hdl.handle.net/10532/2127
dc.language.isoenes_ES
dc.relation.datahttp://www.actahort.org/books/932/932_20.htmes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/es/*
dc.subject.agrovocPrunus salicinaes
dc.subject.agrovocAutoesterilidades
dc.subject.agrovocElectroforesises
dc.subject.agrovocGenotiposes
dc.subject.otherfruticulturaes_ES
dc.titleS-genotyping in Japanese plum by PCR and capillary gel electrophoresis detectionen
dc.typearticle*
dc.type.refereedNon-Refereedes_ES
dc.type.specifiedArticlees_ES

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