Relationship between ultrasonic properties and structural changes in the mesophyll during leaf dehydration
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Date
2011
Authors
Gil Pelegrín, EustaquioSancho Knapik, Domingo
Gómez Álvarez Arenas, Tomás E.
Peguero Pina, José Javier
Fernández, V.
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articleArticle
Abstract
The broad-band ultrasonic spectroscopy technique allows the determination of changes in the relative water content (RWC) of leaves with contrasting structural features. Specifically, the standardized frequency associated with the maximum transmittance (f/fo) is strongly related to the RWC. This relationship is characterized by the existence of two phases separated by an inflexion point (associated with the turgor loss point). To obtain a better understanding of the strong relationship found between RWC and f/fo, this work has studied the structural changes experienced by Quercus muehlenbergii leaves during dehydration in terms of ultrasounds measurements, cell wall elasticity, leaf thickness, leaf density, and leaf structure. The results suggest that the decrease found in f/fo before the turgor loss point can be attributed to the occurrence of changes in the estimation of the macroscopic effective elastic constant of the leaf (c33), mainly associated with changes in the bulk modulus of elasticity of the cell wall (?). These changes are overriding or compensating for the thickness decreases recorded during this phase. On the other hand, the high degree of cell shrinkage and stretching found in the mesophyll cells during the second phase seem to explain the changes in the acoustic properties of the leaf beyond the turgor loss point. The formation of large intercellular spaces, which increased the irregularity in the acoustic pathway, may explain the increase of the attenuation coefficient of ultrasounds once the turgor loss point threshold is exceeded. The direct measurement of c33 from ultrasonic measurements would allow a better knowledge of the overall biomechanical properties of the leaf further than those derived from the P-V analysis.
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Bibliographic citation
Sancho-Knapik, D., Álvarez-Arenas, T. G., Peguero-Pina, J. J., Fernández, V., & Gil-Pelegrín, E. (2011). Relationship between ultrasonic properties and structural changes in the mesophyll during leaf dehydration. Journal of Experimental Botany, 62(10), 3637-3645. https://doi.org/10.1093/jxb/err065
AGROVOC subjects
Pared celularHojas
Contenido de agua
Turgencia
Potencial hídrico foliar
Other field subjects
AirArticle
Cell Wall
Cell wall elasticity
Cryoelectron Microscopy
Dehydration
Drought
leaf thickness
MEMBRANE FILTERS
Metabolism
Microscopy, Electron, Scanning
Plant Leaf
Plant Leaves
Plants
Quercus muehlenbergii
relative water content
Scanning Electron Microscopy
Spectroscopy
Stomatal Closure
Thickness
turgor loss point
Turgor pressure
Ultrasonic spectroscopy
Ultrasonics
Ultrasound
Water
Water potential
Water status
Sponsorship
Este estudio ha sido parcialmente financiado por el proyecto SUM2008-00004-C03-03 del INIA (Ministerio de Ciencia e Innovación). También se agradece el apoyo financiero del Gobierno de Aragón (grupo de investigación A54).