Blood transcriptome of Rasa Aragonesa rams with different sexual behavior phenotype reveals CRYL1 and SORCS2 as genes associated with this trait

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dc.bibliographicCitation.endpage8es_ES
dc.bibliographicCitation.stpage1es_ES
dc.bibliographicCitation.titleJournal Of Animal Scienceen
dc.bibliographicCitation.volume101es_ES
dc.contributor.authorLakhssassi, Kenzaes_ES
dc.contributor.authorSarto Aured, María Pilares_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.contributor.authorSerrano, Malenaes_ES
dc.contributor.authorCalvo Lacosta, Jorge Hugoes_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2025-02-10T08:41:37Z
dc.date.available2025-02-10T08:41:37Z
dc.date.issued2023es_ES
dc.date.updated2025-02-06T08:46:50Z
dc.description.abstractReproductive fitness of rams is seasonal, showing the highest libido during short days coinciding with the ovarian cyclicity resumption in the ewe. However, the remarkable variation in sexual behavior between rams impair farm efficiency and profitability. Intending to identify in vivo sexual behavior biomarkers that may aid farmers to select active rams, transcriptome profiling of blood was carried out by analyzing samples from 6 sexually active (A) and 6 nonactive (NA) Rasa Aragonesa rams using RNA-Seq technique. A total of 14,078 genes were expressed in blood but only four genes were differentially expressed (FDR1) in active rams, whereas the CRYL1 and immunoglobulin lambda-1 light chain isoform X47 (ENSOARG00020025518) genes were downregulated (log2FC < -1) in this same group. Gene set Enrichment Analysis (GSEA) identified 428 signaling pathways, predominantly related to biological processes. The lysosome pathway (GO:0005764) was the most enriched, and may affect fertility and sexual behavior, given the crucial role played by lysosomes in steroidogenesis, being the SORCS2 gene related to this signaling pathway. Furthermore, the enriched positive regulation of ERK1 and ERK2 cascade (GO:0070374) pathway is associated with reproductive phenotypes such as fertility via modulation of hypothalamic regulation and GnRH-mediated production of pituitary gonadotropins. Furthermore, external side of plasma membrane (GO:0009897), fibrillar center (GO:0001650), focal adhesion (GO:0005925), and lamellipodium (GO:0030027) pathways were also enriched, suggesting that some molecules of these pathways might also be involved in rams’ sexual behavior. These results provide new clues for understanding the molecular regulation of sexual behavior in rams. Further investigations will be needed to confirm the functions of SORCS2 and CRYL1 in relation to sexual behavior.en
dc.description.othersexual behavioren
dc.description.othertranscriptomeen
dc.description.otherramsen
dc.description.otherblooden
dc.description.otherCRYL1en
dc.description.otherSORCS2en
dc.description.sponsorshipThis study was partially financed by the Ministry of Education and Science of Spain research project INIA-RTA2015-00090, the Research Group Funds of the Aragón Government (Ref. A14_20R), and FEDER funds. K. Lakhssassi is supported by a doctoral (BES-2017-080154) grant.es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationLakhssassi, K.; Sarto, M. P.; Lahoz, B.; Alabart, J.L.; Folch, J.; Serrano, M. ; Calvo, J.J. Blood transcriptome of Rasa Aragonesa rams with different sexual behavior phenotype reveals CRYL1 and SORCS2 as genes associated with this trait . Journal Of Animal Science, 2023, 101, skad098-
dc.identifier.issn00218812
dc.identifier.urihttp://hdl.handle.net/10532/7479
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1093/jas/skad098es_ES
dc.relation.urihttps://doi.org/10.1093/jas/skad098es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocComportamiento sexuales
dc.subject.agrovocCorderoes
dc.subject.agrovocGeneses
dc.subject.agrovocTranscriptomases
dc.subject.agrovocFenotiposes
dc.subject.agrovocRazas autóctonases
dc.subject.otherAnimals
dc.subject.otherBlood
dc.subject.otherCRYL1
dc.subject.othercrystallins
dc.subject.otherExpression
dc.subject.otherFemale
dc.subject.otherHORMON
dc.subject.otherMale
dc.subject.otherModel
dc.subject.otherPhenotype
dc.subject.otherRAMS
dc.subject.otherReceptors, Cell Surface
dc.subject.otherReproduction
dc.subject.otherSexual Behavior
dc.subject.otherSexual Behavior, Animal
dc.subject.otherSheep
dc.subject.otherSheep, Domestic
dc.subject.otherSORCS
dc.subject.otherSORCS2
dc.subject.otherTranscriptome
dc.titleBlood transcriptome of Rasa Aragonesa rams with different sexual behavior phenotype reveals CRYL1 and SORCS2 as genes associated with this traiten
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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