Desarrollo y evaluación de cuatro PCRs para la detección de Salmonella en ganglios linfáticos porcinos
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Date
2011
Authors
Sánchez, S.San Román, Beatriz
Garrido, Victoria
Abuelo, A.
Asensio, A.C.
Vico, Juan Pablo
Mainar Jaime, Raúl Carlos
Grilló Dolset, María Jesús
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conferenceObjectPaper
Abstract
Salmonellosis is one of the major zoonoses worldwide extended, frequently
transmitted by subclinically infected pigs. Thus, EU health authorities have initiated
salmonellosis control programs including prevalence studies in pigs by microbiological
culture of lymph nodes (LN) following the ISO 6579 method. This method has some
disadvantages that recommend the development of new diagnostic tools. The aim of this
study was to develop and evaluate the usefulness of new PCRs for detecting Salmonella in
LN pig samples, by amplifying 2 chromosomal (A and B) and 2 plasmidic (C and D) genes
highly conserved in Salmonella. These PCRs were evaluated (i) in lab conditions; (ii) against
38 contaminants; (iii) against 132 Salmonella strains of 37 serotypes; and (iv) with 320 swine
LN samples. Results shown that: (i) the four PCRs were specific for Salmonella; (ii) PCR-A
and PCR-B had more sensitivity than PCR-C and PCR-D, unrelated to the serotype; (iii)
PCR-A showed 94.4% SeR vs. the ISO method, while it was below 63.6% for the other
genes; and (iv) best results of SeR was obtained by considering the 4 PCRs jointly, allowing
to detect Salmonella in 26 ISO- samples, 17 (65.4%) of which resulted positive after a
second culture. In conclusion, the combined PCRs showed higher diagnostic sensitivity,
indicating its usefulness as a complementary or alternative method to the ISO.
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Keywords
Salmonella, Swine, Lymph nodes, Diagnosis, Sensitivity
Bibliographic citation
Sánchez, S., San Román, Garrido, V., B., Abuelo, A., Asensio, A.C., Vico, J.P., Mainar-Jaime, R.C., Grilló, M.J. “Desarrollo y evaluación de cuatro PCRs para la detección se Salmonella en ganglios linfáticos porcinos”. En: 41 Jornadas de Estudio. XIV Jornadas sobre Producción Animal. AIDA. Zaragoza, España, 17 y 18 de mayo de 2011, pp.801-803. ISBN: 978-84-615-0062-8
Other field subjects
SalmonelosisCerdo
Control de enfermedades
Producción y sanidad animal