Identification of Self-Incompatibility Alleles by Specific PCR Analysis and S-RNase Sequencing in Apricot
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Date
2018
Authors
Herrera Lagranja, SaraRodrigo García, Francisco Javier
Hormaza Urroz, José Ignacio
Lora, Jorge
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Abstract
Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-Incompatibility (GSI) system. Incompatibility relationships between cultivars can be established
by an S-allele genotyping PCR strategy. Until recently, most of the traditional European apricot cultivars were self-compatible but several breeding programs have introduced an increasing number of new cultivars whose pollination requirements are unknown. To fill this gap, we have identified the S-allele of 44 apricot genotypes, of which 43 are reported here for the first time.
The identification of Sc in 15 genotypes suggests that those cultivars are self-compatible. In five genotypes, self-(in)compatibility was established by the observation of pollen tube growth in self-pollinated flowers, since PCR analysis could not allowed distinguishing between the Sc and S8 alleles. Self-incompatible genotypes were assigned to their corresponding self-incompatibility groups.
The knowledge of incompatibility relationships between apricot cultivars can be a highly valuable tool for the development of future breeding programs by selecting the appropriate parents and for efficient orchard design by planting self-compatible and inter-compatible cultivars
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International Journal Molecular Science, 2018,vol. 19 p. 3612
AGROVOC subjects
AutocompatibilidadPrunus armeniaca
Polen
Polinización
Albaricoque
Sponsorship
This research was funded by Ministerio de Ciencia, Innovación y Universidades—European Regional Development Fund, European Union (AGL2016-77267-R, and AGL2015-74071-JIN); Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (RFP2015-00015-00, RTA2014-00085-00, RTA2017-00003-00);
Gobierno de Aragón—European Social Fund, European Union (Grupo Consolidado A12_17R), Fundación Biodiversidad, and Agroseguro S.A.