Molecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones

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dc.bibliographicCitation.issue11es_ES
dc.bibliographicCitation.stpage2619es_ES
dc.bibliographicCitation.titleAgronomy-Baselen
dc.bibliographicCitation.volume14es_ES
dc.contributor.authorBalduque Gil, Joaquínes_ES
dc.contributor.authorGarcés Claver, Anaes_ES
dc.contributor.authorPérez Lamuela, Inéses_ES
dc.contributor.authorBarriuso Vargas, Juan J.es_ES
dc.contributor.authorFayos Avellán, Oretoes_ES
dc.date.accessioned2024-11-21T12:38:21Z
dc.date.available2024-11-21T12:38:21Z
dc.date.issued2024es_ES
dc.date.updated2024-11-08T11:18:21Z
dc.description.abstractGrapevine (Vitis vinifera L.) varieties are particularly susceptible to the pathogens downy mildew Plasmopara viticola and powdery mildew Erysiphe necator. Conventional methods for identifying and classifying spores rely on time-consuming microscopic examinations susceptible to human error and requiring qualified personnel. The aim of the present work has focused on the establishment of a protocol for the rapid molecular detection of the fungal species P. viticola and E. necator from adhesive tapes used to trap spores in airborne inoculum collector cyclones. Four DNA extraction methods were tested. Subsequently, molecular detection of both pathogens was performed by validating some of the specific molecular markers available in the literature. PCR with the primers Nad9 cob-F/Nad9 cob-R and Uncin144/Uncin511 showed specific results for P. viticola and E. necator, respectively, and the best results were obtained with the T-CTAB method. The methodology developed in this work could be of great help for relating direct measurement of P. viticola and E. necator airborne inoculum to disease risk and detection of pathogens, which could be integrated into the early diagnosis of these grapevine pathogens, improving existing warning systems such as Decision Support Systems.en
dc.description.otherIntegrated Pest Managementen
dc.description.otherearly detectionen
dc.description.otherDNA extractionen
dc.description.othermolecular markersen
dc.description.sponsorshipLos autores agradecen el apoyo de los fondos del Gobierno de Aragón a los grupos de investigación A11-20R y A08-20R.es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationBalduque-Gil, J., Garcés-Claver, A., Pérez-Lamuela, I., Barriuso-Vargas, J. J., & Fayos, O. (2024). Molecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclones. Agronomy, 14(11), Article 11. https://doi.org/10.3390/agronomy14112619
dc.identifier.issn2073-4395
dc.identifier.urihttp://hdl.handle.net/10532/7367
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.3390/agronomy14112619es_ES
dc.relation.urihttps://doi.org/10.3390/agronomy14112619es_ES
dc.rightsAttribution 4.0 International (CC BY 4.0)es_ES
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.agrovocGestión de lucha integradaes
dc.subject.agrovocDetecciónes
dc.subject.agrovocMarcadores genéticoses
dc.subject.agrovocEsporases
dc.subject.otherDetección
dc.subject.otherEsporas
dc.subject.otherGestión De Lucha Integrada
dc.subject.otherMarcadores genéticos
dc.titleMolecular Detection of the Grapevine Pathogens Plasmopara viticola and Erysiphe necator from Airborne Inoculum Collector Cyclonesen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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