Assessing Field Prunus Genotypes for Drought Responsive Potential by Carbon Isotope Discrimination and Promoter Analysis
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Date
2018
Authors
Bielsa Pérez, BeatrizBasset, Carole
Glenn, D. Michael
Rubio Cabetas, María José
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Abstract
In order to improve the effectiveness of breeding practices for Prunus rootstocks, it is
essential to obtain new resistance resources, especially with regard to drought. In this study,
a collection of field-grown Prunus genotypes, both wild-relative species and cultivated hybrid
rootstocks, were subjected to leaf ash and carbon isotope discrimination (∆
13C) analyses, which are
strongly correlated to water use efficiency (WUE). Almond and peach wild relative species showed the
lowest ∆
13C ratios, and therefore, the highest WUE in comparison with hybrid genotypes. In addition,
drought-related cis-regulatory elements (CREs) were identified in the promoter regions of the effector
gene PpDhn2, and the transcription factor gene DREB2B, two genes involved in drought-response
signaling pathways. The phylogenetic analysis of these regions revealed variability in the promoter
region sequences of both genes. This finding provides evidence of genetic diversity between the
peach- and almond-relative individuals. The results presented here can be used to select Prunus
genotypes with the best drought resistance potential for breeding.
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Bibliographic citation
Agronomy, 8(4), (2018)
AGROVOC subjects
PrunusPrunus persica
Variedades
Rizomas
Eficacia en el uso del agua