Changes in HSP gene and protein expression in natural scrapie with brain damage
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Date
2011
Authors
Serrano, CarmenBolea, Rosa
Lyahyai, Jaber
Filali, Hicham
Varona, Luis
Marcos Carcavilla, Ane
Acín, Cristina
Calvo Lacosta, Jorge Hugo
Serrano, Mª Magdalena
Badiola, Juan J.
Zaragoza Fernández, María Pilar
Martín Burriel, Inmaculada
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ArticleAbstract
Heat shock proteins (Hsp) perform cytoprotective functions such as apoptosis regulation and inflammatory
response control. These proteins can also be secreted to the extracellular medium, acting as inflammatory
mediators, and their chaperone activity permits correct folding of proteins and avoids the aggregation of
anomalous isoforms. Several studies have proposed the implication of Hsp in prion diseases. We analysed the gene
expression and protein distribution of different members of the Hsp27, Hsp70, and Hsp90 families in the central
nervous system of sheep naturally infected with scrapie. Different expression profiles were observed in the areas
analysed. Whereas changes in transcript levels were not observed in the cerebellum or medulla oblongata, a
significant decrease in HSP27 and HSP90 was detected in the prefrontal cortex. In contrast, HSP73 was overexpressed
in diencephalons of scrapie animals. Western blotting did not reveal significant differences in Hsp90 and
Hsp70 protein expression between scrapie and control animals. Expression rates identified by real-time RT-PCR and
western blotting were compared with the extent of classical scrapie lesions using stepwise regression. Changes in
Hsp gene and protein expression were associated with prion protein deposition, gliosis and spongiosis rather than
with apoptosis. Finally, immunohistochemistry revealed intense Hsp70 and Hsp90 immunolabelling in Purkinje cells
of scrapie sheep. In contrast, controls displayed little or no staining in these cells. The observed differences in gene
expression and protein distribution suggest that the heat shock proteins analysed play a role in the natural form of
the disease.
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Bibliographic citation
Serrano, C. [et al.]. Changes in HSP gene and protein expression in natural scrapie with brain damage. Veterinary Research 2011, 42:13, 12 p.
Other field subjects
Enfermedades de los animalesVariación genética
Encefalopatía espongiforme
Proteínas de shock térmico
Enfermedades de priones
Ovinos
Apoptosis
Sistema nervioso central
Scrapie
Respuesta inmunológica
Producción y sanidad animal