Activity of Geranium macrorrhizum extracts, essential oils and their main components against three relevant flagellated protozoa

Simple item page
dc.contributor.authorMarcos Herraiz, Sara
dc.contributor.authorIrisarri Gutiérrez, Maria Jose
dc.contributor.authorCarrión, Javier
dc.contributor.authorAzami Conesa, Iris
dc.contributor.authorSuárez Lombao, Rodrigo
dc.contributor.authorNavarro Rocha, Juliana
dc.contributor.authorQuílez, Jose Francisco
dc.contributor.authorFernández Barrero, Alejandro
dc.contributor.authorOchoa, Eneko
dc.contributor.authorGonzález Coloma, Azucena
dc.contributor.authorGómez Muñoz, María Teresa
dc.contributor.authorBailén, María
dc.contributor.funderAgencia Estatal de Investigación
dc.contributor.orcidNavarro Rocha, Juliana [0000-0001-7975-9340]
dc.date.accessioned2025-11-07T08:24:58Z
dc.date.available2025-11-07T08:24:58Z
dc.date.issued2025-10-03
dc.date.updated2025-10-07T07:06:23Z
dc.description.abstractPlant-derived natural products are structurally diverse secondary metabolites that play essential ecological roles and have long been recognized for their therapeutic potential. Geranium macrorrhizum is known to produce bioactive extracts and essential oils rich in monoterpenoids and sesquiterpenes. While various Geranium species have demonstrated antiprotozoal activity, the potential of G. macrorrhizum against protozoan parasites remains unexplored. Given the increasing resistance of protozoan pathogens to conventional treatments, there is a pressing need to identify alternative therapeutic agents from natural sources. Plant material was grown in greenhouse and aeroponic systems. Extracts were obtained via hydrodistillation and Soxhlet, and analyzed by GC-MS. In vitro assays were performed to evaluate antiparasitic activity and cytotoxicity. Morphological changes induced by germacrone were analyzed using light microscopy. The study evaluated the antiparasitic potential of essential oils, extracts, and compounds from Geranium macrorrhizum, cultivated under controlled conditions, against Giardia duodenalis, Trichomonas gallinae, and Leishmania infantum. Results showed broad-spectrum activity, particularly from non-polar extracts and EOs, suggesting that lipophilic compounds, especially germacrone, are key contributors to the observed effects. Germacrone exhibited strong activity and selective toxicity. Structural analogues of germacrone showed reduced efficacy, highlighting the importance of its specific chemical structure. Morphological analyses revealed parasite-specific responses to germacrone, contrasting with the more destructive effects of metronidazole and amphotericin B. The findings support G. macrorrhizum as a promising source of natural antiprotozoal agents and justify further investigation into its bioactive compounds and mechanisms of action.
dc.description.peerreviewedNo
dc.description.sponsorshipEste trabajo ha sido financiado parcialmente por las subvenciones PID2020-114207RB-I00 (Ministerio de Ciencia e Innovación de España), MINISTERIO DE CIENCIA, PID2019-106222RB-C32/SRA (Agencia Estatal de Investigación, 10.13039/501100011033), PID2019-106222RB-C31/SRA (Agencia Estatal de Investigación, 10.13039/501100011033) y Unidad Asociada UGR-CSIC BIOPLAG.
dc.identifier.citationMarcos-Herraiz, S., Irisarri-Gutiérrez, M. J., Carrión, J., Conesa, I. A., Lombao, R. S., Navarro-Rocha, J., Quilez, J. F., Barrero, A. F., Larrigan, E. O., González-Coloma, A., Muñoz, M. T. G., & Bailén, M. (2025). Activity of Geranium macrorrhizum extracts, essential oils and their main components against three relevant flagellated protozoa (p. 2025.10.03.680318). bioRxiv. https://doi.org/10.1101/2025.10.03.680318
dc.identifier.doi10.1101/2025.10.03.680318
dc.identifier.issn2692-8205
dc.identifier.urihttps://hdl.handle.net/10532/7956
dc.language.isoen
dc.publisherCold Spring Harbor Laboratory
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.agrovocTrichomonas
dc.subject.agrovocGiardia
dc.subject.agrovocLeishmania
dc.subject.agrovocEsencia (aceite esencial)
dc.subject.agrovocMetabolito secundario
dc.subject.sdgSalud y bienestar
dc.subject.sdgProducción y consumo responsables
dc.subject.sdgVida de ecosistemas terrestres
dc.titleActivity of Geranium macrorrhizum extracts, essential oils and their main components against three relevant flagellated protozoa
dc.typetexto
dc.typeartículo preliminar

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
10242566.pdf
Size:
1.19 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos científicos, técnicos y divulgativos