Potential of selected essential oils for the control of the european truffle beetle (Leiodes Cinnamomeus)
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Date
2024
Authors
González García, VicenteTapia, Daniel
Sánchez Durán, Sergio
Navarro Rocha, Juliana
Andrés, María Fe
González Coloma, Azucena
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conferenceObjectconference paper
Abstract
The bioactivity of 14 essential oils extracted from semi- industrial pilot plant (table 1), were tested in vitro against one of the most important emergent pests of truffle plantations nowadays, the so-called European Black Truffle beetle Leiodes cinnamomeus in terms of repellent activity and contact toxicity. First, a preliminary screening was conducted using filter paper as carrier in both assays, in order to select the most promising plant species. For this purpose, 0.5% (v/v) and 400µg/insect were the initial doses. From this screening. Mentha rotundifolia, Satureja montana. Tanacetum vulgare and Origanum virens were the most effective in contact toxicity (100%); regarding repellent activity, this effect was observed, with a certain knock- down effect in most of the species chosen due to neurotoxicity of essential oils. In the light of these results, a follow-up toxicity dose-response experiment was undergone for filter paper formulation, in parallel with different formulations (alginate encapsulation and application on cellulose microfiber dishcloth) of the above mentioned 4 essential oils for the same doses. Activity assessed on cellulose microfiber dishcloths remained the highest after lowering doses to 100µg/insect. Also, another follow-up experiment was designed to better asses repellent activity. Dose was doubled in such oils where the knock-down was the lightest and halved in those where the knock-down was stronger. Nearly every oil saw its repellent activity enhanced following this strategy, thus dose-response experiments were undergone in those where the knock-down had been the greater. Again, the same 4 essential oils returned the best bioactivity and were diluted to 1/10 of the original dose. The same alternative formulations as for the toxicity assays were tested for such 4 essential oils, with remarkable differences in the bioactivity presented.
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Bibliographic citation
González, V., Tapia, D., Sánchez-Durán, S., Navarro, J., Navarro, J., Andrés, M.F. & González-Coloma, A. (2024) Potential of selected essential oils for the control of the european truffle beetle (Leiodes Cinnamomeus). En: 20th International Plant Protection Congress. Abstract Book, 2024, 157-157
AGROVOC subjects
Esencias (aceites esenciales)Repelentes de plagas
Toxicidad por contacto
Coleoptera
Tuber melanosporum
Other field subjects
ColeopteraEsencias (Aceites Esenciales)
Repelentes de plagas
Toxicidad Por Contacto
Tuber melanosporum
Sponsorship
La realización de este trabajo ha sido posible gracias al proyecto Nuevas estrategias de control del escarabajo de la truta en Teruel ColeapTE: I+D+ICIBR-FITE 2021 / The realization of this work was possible thanks to the project: Nuevas estrategias de control del escarabajo de la truta en Teruel ColeapTE: I+D+ICIBR-FITE 2021