Amplicon sequencing with Oxford nanopore technologies as a diagnostic alternative for small ruminant lentiviruses in sheep

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dc.contributor.authorSerrano Noreña, Magdalena
dc.contributor.authorGonzález, Carmen
dc.contributor.authorRoy, Rosa
dc.contributor.authorFernandez, Almudena
dc.contributor.authorArranz, Juan José
dc.contributor.authorCalvo Lacosta, Jorge Hugo
dc.contributor.authorJiménez, María Angeles
dc.contributor.authorPuente-Sánchez, Fernando
dc.contributor.orcidCalvo Lacosta, Jorge Hugo [0000-0001-9513-0219]
dc.date.accessioned2026-01-28T12:05:23Z
dc.date.available2026-01-28T12:05:23Z
dc.date.issued2026-01-25
dc.date.updated2026-01-26T09:19:42Z
dc.description.abstractIn Europe, Maedi-Visna disease has high prevalence rates at the individual and flock levels, respectively, and is regarded as one of the most significant infectious disease in sheep. The lack of treatment or a commercial vaccine underscores the need for accurate and reliable diagnostic tools to support control programs. Conventional methods, including ELISA and qPCR, provide useful but incomplete information due to the genetic variability of small ruminant lentiviruses (SRLVs) and the heterogeneous host immune response. In this work, third-generation sequencing was assessed as a diagnostic strategy, focusing on Oxford Nanopore Technologies amplicon sequencing of different regions of the virus genome. DNA from whole blood, PBMCs, semen, and nasal mucosa of 44 rams previously tested for Maedi-Visna virus by ELISA was used to generate amplicons of the gag, pol, and p25 genes. Sequencing showed that blood DNA was the most reliable source for SRLVs detection by Nanopore, despite the low proportion of monocytes present in this medium. Compared with conventional approaches, Nanopore sequencing reduced the proportion of false negatives observed with ELISA (42%) and qPCR (77%). These results highlight Nanopore amplicon sequencing as a promising diagnostic alternative, combining epidemiological relevance with technological innovation to enhance SRLVs detection and strengthen control strategies for sustainable disease management.
dc.description.peerreviewedSi
dc.description.sponsorshipFinanciación de acceso abierto proporcionada gracias al acuerdo CRUE-CSIC con Springer Nature. Este trabajo ha sido cofinanciado por el Ministerio de Ciencia e Innovación de España, el Plan de Investigación Científica, Técnica y de Innovación 2021-2023, el proyecto PID2022-136444OB-C31 y el Fondo Europeo de Desarrollo Regional (FEDER).
dc.identifier.citationSerrano, M., González, C., Roy, R., Fernández, A., Arranz, J. J., Calvo, J. H., Jiménez, M. Á., & Puente-Sánchez, F. (2026). Amplicon sequencing with Oxford nanopore technologies as a diagnostic alternative for small ruminant lentiviruses in sheep. Scientific Reports. https://doi.org/10.1038/s41598-026-36989-y
dc.identifier.urihttps://hdl.handle.net/10532/8118
dc.identifier.urihttps://doi.org/10.1038/s41598-026-36989-y
dc.language.isoen
dc.publisherSpringer Nature Limited
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 4.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/es/
dc.subject.agrovocVisna-maedi virus
dc.subject.agrovocLentivirus
dc.subject.agrovocGenética molecular
dc.subject.agrovocGenotipado SNP
dc.subject.agrovocDiagnóstico
dc.subject.sdgHambre cero
dc.titleAmplicon sequencing with Oxford nanopore technologies as a diagnostic alternative for small ruminant lentiviruses in sheep
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión sometida a revisión

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