Genetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3

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dc.bibliographicCitation.titleFrontiers in Veterinary Scienceen
dc.bibliographicCitation.volume6es_ES
dc.contributor.authorGuzmán Verri, Caterinaes_ES
dc.contributor.authorSuárez Esquivel, Marcelaes_ES
dc.contributor.authorRuiz Villalobos, Nazarethes_ES
dc.contributor.authorZygmunt, Michael Stanislases_ES
dc.contributor.authorGonnet, Mathieues_ES
dc.contributor.authorCampos, Elenaes_ES
dc.contributor.authorVíquez Ruiz, Eunicees_ES
dc.contributor.authorChacón Díaz, Carloses_ES
dc.contributor.authorAragón Aranda, Beatrizes_ES
dc.contributor.authorConde Álvarez, Raqueles_ES
dc.contributor.authorMoriyón Uria, Ignacioes_ES
dc.contributor.authorBlasco Martínez, José Maríaes_ES
dc.contributor.authorMuñoz Álvaro, Pilar Maríaes_ES
dc.contributor.authorBaker, Kate S.es_ES
dc.contributor.authorThomson, Nicholas R.es_ES
dc.contributor.authorCloeckaert, Axeles_ES
dc.contributor.authorMoreno, Edgardoes_ES
dc.coverage.spatialProducción y sanidad animales_ES
dc.date.accessioned2019-07-04T11:35:01Z
dc.date.available2019-07-04T11:35:01Z
dc.date.issued2019es_ES
dc.description.abstractMembers of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as “Brucella melitensis biovar 2”. Further molecular typing, identified the strain as an atypical “Brucella suis”. Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella clade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.en
dc.description.statusPublishedes_ES
dc.identifier.citationFrontiers in Veterinary Science, vol. 6, (2019)
dc.identifier.urihttp://hdl.handle.net/10532/4710
dc.language.isoenes_ES
dc.relation.doi10.3389/fvets.2019.00175es_ES
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fvets.2019.00175/fulles_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.agrovocBrucellaes
dc.subject.agrovocBrucelosises
dc.subject.agrovocBacteriologíaes
dc.titleGenetic and Phenotypic Characterization of the Etiological Agent of Canine Orchiepididymitis Smooth Brucella sp. BCCN84.3en
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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