SNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed.

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dc.bibliographicCitation.endpage72es_ES
dc.bibliographicCitation.stpage63es_ES
dc.bibliographicCitation.titleTheriogenology: An International Journal Of Animal Reproductionen
dc.bibliographicCitation.volume113es_ES
dc.contributor.authorCalvo Lacosta, Jorge Hugoes_ES
dc.contributor.authorSerrano, M.es_ES
dc.contributor.authorMartinez-Royo, A.es_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorSarto, A.es_ES
dc.contributor.authorIbañez-Deler, A.es_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2025-02-10T08:57:56Z
dc.date.available2025-02-10T08:57:56Z
dc.date.issued2018es_ES
dc.date.updated2025-02-06T10:51:19Z
dc.description.abstractThe aim of this study was to characterize and identify causative SNPs in the MTNR1A gene responsible for the reproductive seasonality traits in the Rasa aragonesa sheep breed. A total of 290 ewes (155, 84 and 51 mature, young and ewe lambs, respectively) from one flock were controlled from January to August. The following three reproductive seasonality traits were considered: the total days of anoestrus (TDA) and the progesterone cycling months (P4CM); both ovarian function seasonality traits based on blood progesterone levels; and the oestrus cycling months (OCM) based on oestrous detection, which indicate behavioural signs of oestrous. We have sequenced the total coding region plus 733 and 251 bp from the promoter and 3'-UTR regions, respectively, from the gene in 268 ewes. We found 9 and 4 SNPs associated with seasonality traits in the promoter (for TDA and P4CM) and exon 2 (for the three traits), respectively. The SNPs located in the gene promoter modify the putative binding sites for various trans-acting factors. In exon 2, two synonymous SNPs affect RFLP sites, rs406779174/RsaI (for the three traits) and rs430181568/MnlI (for OCM), and they have been related with seasonal reproductive activity in previous association studies with other breeds. SNP rs400830807, which is located in the 3'-UTR, was associated with the three traits, but this did not modify the putative target sites for ovine miRNAs according to in silico predictions. Finally, the SNP rs403212791 (NW_014639035.1: g.15099004G > A), which is also associated with the three seasonality phenotypes, was the most significant SNP detected in this study and was a non-synonymous polymorphism, leading a change from an Arginine to a Cysteine (R336C). Haplotype analyses confirmed the association results and showed that the effects found for the seasonality traits were caused by the SNPs located in exon 2. We have demonstrated that the T allele in the SNP rs403212791 in the MNTR1A gene is associated with a lower TDA and higher P4CM and OCM values in the Rasa Aragonesa breed.Copyright © 2018 Elsevier Inc. All rights reserved.en
dc.description.othersheepen
dc.description.otheroestrous behaviouren
dc.description.otherovulatory activityen
dc.description.otherMTNR1Aen
dc.description.otherSNPen
dc.description.sponsorshipThis study was partially financed by the Ministry of Economy and Competitiveness research projects INNPACTO IPT-010000-2010-33 and INIA-RTA2013-00041 and the European Union Regional Development funds (and the Research Group Funds of the Aragón Government, A13 and A49). We would like to thank Grupo Pastores for providing the experimental animals and facilities for the study.es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationCalvo, J.H.; Serrano, M.; Martinez-Royo, A.; Lahoz, B.; Sarto P.; Ibañez- Deler, A.; Folch, J.; Alabart, J.L. SNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed, Theriogenology, 2018, 113, 63-72
dc.identifier.issn0093691X
dc.identifier.urihttp://hdl.handle.net/10532/7480
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1016/j.theriogenology.2018.02.013es_ES
dc.relation.urihttps://doi.org/10.1016/j.theriogenology.2018.02.013es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocDetección del estroes
dc.subject.agrovocCorderoes
dc.subject.agrovocIntestino Caudales
dc.subject.agrovocOvotestises
dc.subject.agrovocReproducción animales
dc.subject.agrovocRazas autóctonases
dc.subject.otherAnimals
dc.subject.otherDetección Del Estro
dc.subject.otherExpression
dc.subject.otherFLOCK
dc.subject.otherGene Expression Regulation
dc.subject.otherHaplotypes
dc.subject.otherLinkage Disequilibrium
dc.subject.otherMelatonin
dc.subject.otherMELATONIN-RECEPTOR-1A
dc.subject.otherMTNR1A
dc.subject.otherOestrous behaviour
dc.subject.otherOvulatory activity
dc.subject.otherPolymorphism
dc.subject.otherPolymorphism, Single Nucleotide
dc.subject.otherPromoter Regions, Genetic
dc.subject.otherRECEPTOR 1A GENE
dc.subject.otherReceptor, Melatonin, MT1
dc.subject.otherReproduction
dc.subject.otherResponses
dc.subject.otherSARDA EWES
dc.subject.otherSeasons
dc.subject.otherSheep
dc.subject.otherSnp
dc.titleSNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed.en
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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