Diagnosis and detection of the main bacterial pathogens of stone fruit and almond
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Date
2010
Authors
López González, María MilagrosRoselló Pérez, Montserrat
Palacio Bielsa, Ana
Journal Title
Journal ISSN
Volume Title
Publisher
Typology
ArticleAbstract
Diagnosis and detection are key aspects related to plant health status. A critical review of the available diagnostic methods utilised for Agrobacterium rhizogenes and A. tumefaciens, Pseudomonas amygdali, P. yringae
pv. mors-prunorum, P. syringae pv. persicae, P. syringae pv. syringae and Xanthomonas arboricola pv. pruni, the main pathogens of the stone fruit trees, is presented. As
there is a general lack of updated standardized protocols for the detection of most of these bacteria, the most appropriate media for their isolation are reported along
with serological reagents, PCR and real-time PCR protocols with comments on their accuracy for the analysis of these pathogens in plant samples. There are many selective
media for isolation, especially for Agrobacterium
spp., but fewer for Pseudomonas spp. and X. arboricola pv. pruni. Serological techniques are not very useful for these pathogens due to the current lack of specific antibodies
commercially available. As to molecular methods, it is surprising to find so many PCR protocols for Agrobacterium species, very few and unspecific for the Pseudomonas species pathogenic to stone fruit trees,
and several recent PCR protocols for X. arboricola pv. pruni. The new advances in genomics and proteomics will provide information for selecting new targets to develop specific and sensitive techniques for the diagnosis and detection of these bacterial pathogens in plant material.
Description
Keywords
Isolation, Selective media, PCR, Real-time PCR, Immunofluorescence, ELISA
Bibliographic citation
López, M.M.; Roselló, M.; Palacio Bielsa, A. "Diagnosis and detection of the main bacterial pathogens of stone fruit and almond". Journal of plant pathology, 2010, vol. 92, suplemento 1, p. s57-s66
Other field subjects
Frutas de huesoPrunus
Organismos patógenos
Sanidad vegetal