Identification of ShgH as a dual histidine/glutamine transporter component essential for Streptococcus suis virulence and biofilm modulation

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dc.contributor.authorGarcía, Carla
dc.contributor.authorSaralegui Remón, Luis
dc.contributor.authorMorales Lasierra, Beatriz
dc.contributor.authorJurado Romero, Paula
dc.contributor.authorBes Fustero, Teresa
dc.contributor.authorMarín Alcalá, Clara María
dc.contributor.authorArenas Busto, Jesús
dc.contributor.funderAgencia Estatal de Investigación
dc.contributor.orcidMarín Alcalá, Clara María [0000-0002-1974-9025]
dc.date.accessioned2025-11-06T15:54:35Z
dc.date.available2025-11-06T15:54:35Z
dc.date.issued2025
dc.date.updated2025-10-07T08:01:43Z
dc.description.abstractStreptococcus suis is a zoonotic pathogen that affects pigs and humans. In this study, we characterised ShgH, a predicted substrate-binding component of an ABC transporter. Immunoassays confirmed that ShgH is expressed, secreted and surface-exposed in S. suis, in agreement with its proposed transporter function. Isothermal titration calorimetry demonstrated that ShgH binds glutamine and histidine, with a higher affinity for histidine. Deletion of the shgH gene significantly impaired uptake of both radiolabelled amino acids confirming its role as part of a transporter. Functional analysis revealed that shgH deletion results in a marked reduction in virulence in a murine infection model, while host colonization remained unaffected. ShgH contributes to infection by facilitating evasion of phagocytosis and resistance to oxidative stress through impaired nutrient acquisition and reduced capsule production. In addition, ShgH regulates biofilm formation and architecture. Notably, ShgH is highly conserved among pathogenic streptococci, suggesting a broader functional relevance. Altogether, our findings identify ShgH as a dual glutamine/histidine- binding protein essential for nutrient uptake and virulence in S. suis, and a promising target for future therapeutic interventions.
dc.description.peerreviewedSi
dc.description.sponsorshipEste trabajo ha sido financiado por Ciencia e Innovación/Agencia Española de Investigación MCIN/AEI/10.13039/501100011033 y, según corresponda, por el FEDER «Una Europa más solidaria» de la Unión Europea o por la Unión Europea NextGenerationEU/PRTR (Acuerdo de subvención PID2020-114617RB-100)
dc.identifier.citationGarcía, C., Saralegui, L., Morales, B., Jurado, P., Bes, M. T., Marín, C., & Arenas, J. (2026). Identification of ShgH as a dual histidine/glutamine transporter component essential for Streptococcus suis virulence and biofilm modulation. Microbiological Research, 302, 128354. https://doi.org/10.1016/j.micres.2025.128354
dc.identifier.doi10.1016/j.micres.2025.128354
dc.identifier.issn0944-5013
dc.identifier.urihttps://hdl.handle.net/10532/7952
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.agrovocStreptococcus suis
dc.subject.agrovocVirus patógenos
dc.subject.agrovocSecuencia de aminoácidos
dc.subject.agrovocBiofilm (microbiología)
dc.subject.agrovocZoonosis
dc.subject.sdgSalud y bienestar
dc.subject.sdgProducción y consumo responsables
dc.subject.sdgVida de ecosistemas terrestres
dc.titleIdentification of ShgH as a dual histidine/glutamine transporter component essential for Streptococcus suis virulence and biofilm modulation
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión publicada

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