The use of meat juice or blood serum for the diagnosis of Salmonella infection in pigs and its possible implications on Salmonella control programs
Loading...
Date
2011
Authors
Vico, Juan PabloMainar Jaime, Raúl Carlos
Journal Title
Journal ISSN
Volume Title
Publisher
Typology
ArticleAbstract
Serology is the method of choice for country-scale Salmonella control programs in pigs and can be carried out
both on blood serum or meat juice. However, the diagnostic performance of enzyme-linked immunosorbent assays (ELISAs)
on these sample matrices has not been sufficiently compared. The agreement between the serum ELISA and meat juice ELISA
on samples taken from commercial farms was assessed in 2 pig populations (adult sows and finishers). Results of optical
density percentage (OD%) from the serum ELISA were consistently higher than those from the meat juice ELISA (38.5
vs. 27.9; P < 0.001), and the mean difference between them was significantly different from zero (P < 0.0001). The overall
correlation coefficient between serum ELISA and meat juice ELISA results was low (r = 0.53). These results indicated an
important disagreement between ELISA performed on serum and meat juice matrices and suggested that before implementing
a control program to reduce the prevalence of Salmonella in swine the choice of matrix on which to perform the ELISA should
be carefully considered
Description
Keywords
Enzyme-linked immunosorbent assay, Meat juice, Pigs, Salmonella, Serum, Test agreement
Bibliographic citation
Juan P. Vico, Raúl C. Mainar Jaime. "The use of meat juice or blood serum for the diagnosis of Salmonella infection in pigs and its possible implications on Salmonella control programs". Journal of Veterinary Diagnostic Investigation. (2011), vol. 23, nº 3, p. 528-531
Other field subjects
Carne de cerdoELISA
Vigilancia de enfermedades
Producción y sanidad animal