Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma

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dc.bibliographicCitation.endpage513es_ES
dc.bibliographicCitation.stpage509es_ES
dc.bibliographicCitation.titleTheriogenology: An International Journal Of Animal Reproductionen
dc.bibliographicCitation.volume81es_ES
dc.contributor.authorAndueza Urra, Jesús Donatoes_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorMuñoz Pérez, Fernandoes_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2025-01-28T08:00:16Z
dc.date.available2025-01-28T08:00:16Z
dc.date.issued2014es_ES
dc.date.updated2025-01-27T10:57:07Z
dc.description.abstractThe objective of this study was to evaluate the ability of near-infrared reflectance spectroscopy (NIRS) to discriminate between pregnant and nonpregnant ewes in early stages of pregnancy after artificial insemination (AI) from blood plasma. Samples were collected using jugular puncture at 18 and 25 days after AI from 188 Rasa Aragonesa and Ansotana ewes. Plasma samples were analyzed for pregnancy-associated glycoprotein (PAG) and progesterone (P4) using ELISA commercial kits. The spectra of plasma samples were recorded in the visible and near-infrared ranges. The performance of these tests were compared, using as criterion standard the pregnancy status determined using transabdominal ultrasonography at 45 days after AI. Pregnancy rate was 47.9% (90/188). At Day 18, sensitivity was similar in NIRS and P4 tests (98.9% vs. 100%; not significant) and greater than PAG (32.2%; both P < 0.001). Specificity was similar in NIRS and PAG tests (both 100%) and greater than that of P4 (84.7%; P < 0.001). At Day 25, sensitivity and specificity of NIRS and PAG were both 100%. It can be concluded that NIRS was an accurate method of diagnosis of pregnancy at Days 18 and 25 after AI in ewes. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.otherEarly pregnancy diagnosisen
dc.description.otherEween
dc.description.otherFertilityen
dc.description.otherNIRSen
dc.description.otherPAGen
dc.description.otherProgesteroneen
dc.description.sponsorshipThis study was funded by the Government of Aragon (Research Group on Improvement of Sheep Production). ELISA kits for pregnancy-associated glycoprotein determination were given by Prof. J.F. Beckers, Université de Liège. The authors thank Pilar Sánchez and María Ángeles Legua for their technical collaboration. An Spanish application patent related to the present research “Procedimiento de identificación de animales gestantes” with application number P201331294 has been filed the 2nd September of 2013es_ES
dc.description.statusSubmittedes_ES
dc.identifier.citationAndueza, D.; Alabart, J. L; Lahoz, B.; Muñoz, F.; Folch, J.Early pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasma. Theriogenology, 2014, 81, 3, 509-513
dc.identifier.issn0093691X
dc.identifier.urihttp://hdl.handle.net/10532/7460
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1016/j.theriogenology.2013.10.016es_ES
dc.relation.urihttps://doi.org/10.1016/j.theriogenology.2013.10.016es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocPreñezes
dc.subject.agrovocOvinoses
dc.subject.agrovocEspectroscopiaes
dc.subject.agrovocPlasma sanguíneoes
dc.subject.agrovocInseminación artificiales
dc.subject.agrovocGestaciónes
dc.subject.otherEarly pregnancy diagnosis
dc.subject.otherEwe
dc.subject.otherFertility
dc.subject.otherNIRS
dc.subject.otherPAG
dc.subject.otherAnimals
dc.subject.otherEarly pregnancy diagnosis
dc.subject.otherEwe
dc.subject.otherFemale
dc.subject.otherFertility
dc.subject.otherNIRS
dc.subject.otherPAG
dc.subject.otherPlasma
dc.subject.otherPregnancy
dc.subject.otherPregnancy tests
dc.subject.otherPregnancy, Animal
dc.subject.otherProgesterone
dc.subject.otherRegression
dc.subject.otherSensitivity and Specificity
dc.subject.otherSheep
dc.subject.otherSpectroscopy, near-infrared
dc.subject.otherTests
dc.titleEarly pregnancy diagnosis in sheep using near-infrared spectroscopy on blood plasmaen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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