Characterisation of the Melatonin Receptor 1A (MTNR1A) gene in the Rasa Aragonesa sheep breed: Association with reproductive seasonality

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dc.bibliographicCitation.endpage175es_ES
dc.bibliographicCitation.issue3-4es_ES
dc.bibliographicCitation.stpage169es_ES
dc.bibliographicCitation.titleAnimal Reproduction Scienceen
dc.bibliographicCitation.volume133es_ES
dc.contributor.authorMartínez Royo, Albertoes_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.contributor.authorCalvo Lacosta, Jorge Hugoes_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2025-01-31T12:51:00Z
dc.date.available2025-01-31T12:51:00Z
dc.date.issued2012es_ES
dc.date.updated2025-01-31T12:47:41Z
dc.description.abstractThe ovine Melatonin Receptor 1A (MTNR1A) gene was structurally characterised and association between its variants and the reproductive seasonality was examined in a daughter design comprising three families of Rasa Aragonesa sheep breed. Sequencing of six Rasa Aragonesa ewes with extreme values for seasonality trait revealed 28 polymorphisms: 11 SNPs in the coding region (all in Exon 2), and 17 SNPs in the promoter region MTNR1A. All the substitutions in the coding region were found most likely lacking any phenotypic effect, because they are conservative mutations or were not part of the transmembrane domain. The silent mutations, which had shown association with reproductive seasonality in other breeds, were also found and genotyped in Rasa Aragonesa. The T allele of SNP606/RsaI of MNTR1A gene was associated with a greater percentage of oestrous cyclic ewes in the Rasa Aragonesa breed, indicating that this SNP may be in linkage disequilibrium with a mutation responsible for this trait close to MTNR1A, or in regulatory sequences of the gene. In this sense, several SNPs affecting a binding element for some transcription factors have been identified in the promoter region. The SNPs at 422 and 527 positions could constitute a binding element for some transcription factors (TFs), located in an EF2 and SRY consensus sites in the promoter region, respectively. Haplotype h5 showed significant differences with the h2 haplotype (66% compared to 49.2%) on oestrous cyclicity, thus these results are consistent with genotypic associations for each SNP. Haplotype with T, A and T alleles for SNPs 422, 677 (promoter region) and 612 (Exon 2) showed an increase of the percentage of oestrous cyclic ewes. Although some of these mutations have been associated with seasonal reproduction, further studies with a more appropriate animal design as well as functional studies of TF binding activity are needed. © 2012 Elsevier B.V.en
dc.description.sponsorshipThis research was funded by the INIA projects RTA2006-0140, TRACE PET-2008-0076, INNPACTO 010000-2010 IPT-33 and co-financed with FEADER funds. Martínez-Royo A. and Lahoz B. received a scholarship from INIA.
dc.description.statusPublishedes_ES
dc.identifier.citationMartínez-Royo, A.; Lahoz, B.; Alabart, J.L., Folch, J.; Calvo, J.H.; Characterisation of the Melatonin Receptor 1A (MTNR1A) gene in the Rasa Aragonesa sheep breed: association with reproductive seasonality,Animal Reproduction Science ,2012, 133, 3-4, 169-175
dc.identifier.issn03784320
dc.identifier.urihttp://hdl.handle.net/10532/7468
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1016/j.anireprosci.2012.06.018es_ES
dc.relation.urihttps://doi.org/10.1016/j.anireprosci.2012.06.018es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Spaines_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocOvinoes_ES
dc.subject.agrovocGestaciónes_ES
dc.subject.agrovocMelatoninaes_ES
dc.subject.agrovocEstacionalidades_ES
dc.subject.agrovocGenotipoes_ES
dc.subject.agrovocReproducción animales_ES
dc.subject.otherAnimal
dc.subject.otherAnimalia
dc.subject.otherAnimals
dc.subject.otherAnoestrus
dc.subject.otherArticle
dc.subject.otherBreeding
dc.subject.otherChromosome Map
dc.subject.otherChromosome Mapping
dc.subject.otherExpression
dc.subject.otherFemale
dc.subject.otherGene Expression Regulation
dc.subject.otherGenetic Polymorphism
dc.subject.otherGenetics
dc.subject.otherGenotype
dc.subject.otherHAPLOTYPE RECONSTRUCTION
dc.subject.otherMelatonin 1 Receptor
dc.subject.otherMetabolism
dc.subject.otherMTNR1A
dc.subject.otherNon-breeding
dc.subject.otherOestrous cyclicity
dc.subject.otherOut of season
dc.subject.otherOvis
dc.subject.otherOvis Aries
dc.subject.otherPhase
dc.subject.otherPhysiology
dc.subject.otherPolymorphism
dc.subject.otherPolymorphism, Genetic
dc.subject.otherPromoter Region
dc.subject.otherPromoter Regions, Genetic
dc.subject.otherRasa Aragonesa
dc.subject.otherReceptor, Melatonin, MT1
dc.subject.otherReproduction
dc.subject.otherResponses
dc.subject.otherSeason
dc.subject.otherSheep
dc.titleCharacterisation of the Melatonin Receptor 1A (MTNR1A) gene in the Rasa Aragonesa sheep breed: Association with reproductive seasonalityen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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