A reliable qPCR technique for detecting viable Xanthomonas arboricola pv. pruni cells

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dc.bibliographicCitation.stpage472es_ES
dc.bibliographicCitation.titleApplied Microbiology And Biotechnologyen
dc.bibliographicCitation.volume108es_ES
dc.contributor.authorSabuquillo, Pilares_ES
dc.contributor.authorBerruete Rodríguez, Isabel Maríaes_ES
dc.contributor.authorCubero, Jaimees_ES
dc.contributor.authorPalacio Bielsa, Anaes_ES
dc.date.accessioned2024-09-26T08:51:09Z
dc.date.available2024-09-26T08:51:09Z
dc.date.issued2024es_ES
dc.date.updated2024-09-26T08:42:52Z
dc.description.abstractXanthomonas arboricola pv. pruni (Xap) is the causal agent of bacterial spot of stone fruits and almond (Prunus spp). Detection of Xap is typically carried out using quantitative real-time PCR (qPCR) combined with culture-based isolation. However, qPCR does not differentiate between viable and dead cells, potentially leading to an overestimation of the infective population in a sample. Such overestimation could result in unnecessary phytosanitary measures. The present study aims to develop a specific protocol ideally targeting to detection of only live Xap bacterial cells. To address this challenge, the viable quantitative PCR (v-qPCR) method was evaluated using three nucleic acid-binding dyes: propidium monoazide (PMA), a combination of PMA and ethidium monoazide (EMA), and PMAxx™, an improved version of PMA. PMAxx™ proved to be the most suitable dye for the detection and quantification of living bacterial cells. This methodology was also evaluated in infected plant material over time and can be considered a rapid and reliable alternative to PCR methods for detecting only those putative infective Xap that may pose a risk for Prunus crops.en
dc.description.otherCut-off Cten
dc.description.otherLODen
dc.description.otherXanthomonas arboricola pv. prunien
dc.description.otherV-qPCRen
dc.description.otherIntercalating dyeen
dc.description.otherViable cell detectionen
dc.description.sponsorshipEste trabajo fue financiado por el proyecto PID2021-123600ORC44, con el apoyo de MICIU/AEI/https://doi.org/10.13039/501100011033 y por el FEDER, UEes_ES
dc.description.statusPublishedes_ES
dc.identifier.citationSabuquillo, P., Berruete, I. M., Cubero, J., & Palacio-Bielsa, A. (2024). A reliable qPCR technique for detecting viable Xanthomonas arboricola pv. Pruni cells. Applied Microbiology and Biotechnology, 108(1), 472. https://doi.org/10.1007/s00253-024-13288-y
dc.identifier.issn01757598
dc.identifier.urihttp://hdl.handle.net/10532/7244
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1007/s00253-024-13288-yes_ES
dc.relation.urihttps://doi.org/10.1007/s00253-024-13288-yes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licensees_ES
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject.agrovocXanthomonas arboricola pv. prunies
dc.subject.agrovocPrunuses
dc.subject.agrovocFrutas de huesoes
dc.subject.agrovocÁrboles frutaleses
dc.subject.agrovocEnfermedades bacterianases
dc.subject.agrovocColoranteses
dc.subject.agrovocReacción en cadena de polimerasa cuantitativaes
dc.subject.agrovocProtocoloses
dc.subject.otherÁrboles frutales
dc.subject.otherColorantes
dc.subject.otherEnfermedades bacterianas
dc.subject.otherFrutas De Hueso
dc.subject.otherProtocolos
dc.subject.otherPrunus
dc.subject.otherReacción en cadena de polimerasa cuantitativa
dc.subject.otherXanthomonas arboricola pv. pruni
dc.titleA reliable qPCR technique for detecting viable Xanthomonas arboricola pv. pruni cellsen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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