Differences in the Ovine HSP90AA1 Gene Expression Rates Caused by Two Linked Polymorphisms at Its Promoter Affect Rams Sperm DNA Fragmentation under Environmental Heat Stress Conditions

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dc.bibliographicCitation.endpage19es_ES
dc.bibliographicCitation.stpage1es_ES
dc.bibliographicCitation.titlePlos Oneen
dc.bibliographicCitation.volume10(2)es_ES
dc.contributor.authorSalces Ortiz, Judites_ES
dc.contributor.authorRamón, Manueles_ES
dc.contributor.authorGonzález, Carmenes_ES
dc.contributor.authorPérez Guzmán, María Doloreses_ES
dc.contributor.authorGarde, J. Juliánes_ES
dc.contributor.authorGarcía Alvarez, Olgaes_ES
dc.contributor.authorMaroto Morales, Alejandroes_ES
dc.contributor.authorCalvo Lacosta, Jorge Hugoes_ES
dc.contributor.authorSerrano Noreña, Magdalenaes_ES
dc.date.accessioned2015-02-24T11:21:44Z
dc.date.available2015-02-24T11:21:44Z
dc.date.issued2015es_ES
dc.description.abstractHeat shock (HS) is one of the best-studied exogenous cellular stresses. Almost all tissues, cell types, metabolic pathways and biochemical reactions are affected in greater or lesser extent by HS. However, there are some especially thermo sensible cellular types such as the mammalian male germ cells. The present study examined the role of three INDELs in conjunction with the -660G/C polymorphism located at the HSP90AA1 promoter region over the gene expression rate under HS. Specially, the -668insC INDEL, which is very close to the -660G/C transversion, is a good candidate to be implied in the transcriptional regulation of the gene by itself or in a cooperative way with this SNP. Animals carrying the genotype II-668 showed higher transcription rates than those with ID-668 (FC = 3.07) and DD-668 (FC = 3.40) genotypes for samples collected under HS. A linkage between gene expression and sperm DNA fragmentation was also found. When HS conditions were present along or in some stages of the spermatogenesis, alternative genotypes of the -668insC and -660G/C mutations are involved in the effect of HS over sperm DNA fragmentation. Thus, unfavorable genotypes in terms of gene expression induction (ID-668GC-660 and DD-668GG-660) do not produce enough mRNA (stored as messenger ribonucleoprotein particles) and Hsp90α protein to cope with future thermal stress which might occur in posterior stages when transcriptional activity is reduced and cell types and molecular processes are more sensible to heat (spermatocytes in pachytene and spermatids protamination). This would result in the impairment of DNA packaging and the consequent commitment of the events occurring shortly after fertilization and during embryonic development. In the short-term, the assessment of the relationship between sperm DNA fragmentation sensitivity and ram’s fertility will be of interest to a better understanding of the mechanisms of response to HS and its consequences on animal production and reproduction performance.en
dc.description.statusPublishedes_ES
dc.identifier.citationPlos One, 10(2), p. 1-19en
dc.identifier.issn1932-6203*
dc.identifier.urihttp://hdl.handle.net/10532/2816
dc.language.isoenes_ES
dc.relation.doi10.1371/journal.pone.0116360es_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/es/*
dc.subject.agrovocOvinoses
dc.subject.agrovocFactores climáticoses
dc.subject.agrovocHerencia genéticaes
dc.subject.agrovocBanco de genotiposes
dc.subject.otherProducción y sanidad animales_ES
dc.titleDifferences in the Ovine HSP90AA1 Gene Expression Rates Caused by Two Linked Polymorphisms at Its Promoter Affect Rams Sperm DNA Fragmentation under Environmental Heat Stress Conditionsen
dc.typeJournal Contribution*
dc.typearticle
dc.type.refereedNon-Refereedes_ES
dc.type.specifiedArticlees_ES

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