Detection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genome

Simple item page
dc.bibliographicCitation.endpage112es_ES
dc.bibliographicCitation.stpage108es_ES
dc.bibliographicCitation.titleScientia Horticulturaeen
dc.bibliographicCitation.volume168es_ES
dc.contributor.authorBielsa Pérez, Beatrizes_ES
dc.contributor.authorJiwan, Des_ES
dc.contributor.authorFernández i Martí, Angel V.es_ES
dc.contributor.authorDhingra, Amites_ES
dc.contributor.authorRubio Cabetas, María Josées_ES
dc.coverage.spatialCiencia vegetales_ES
dc.date.accessioned2025-01-30T11:49:09Z
dc.date.available2025-01-30T11:49:09Z
dc.date.issued2014es_ES
dc.date.updated2025-01-27T11:24:37Z
dc.description.abstractIn order to control tree size, disease, precocity and stress most Prunus varieties are cultivated as composite plants grafted onto desirable rootstocks that impart all the afore-mentioned traits. Several Prunus rootstock breeding programs have been focused on the production of interspecific hybrids. The pedigree of most of these rootstocks remains unknown due to the lack of parental information necessitating the application of DNA-based knowledge in breeding programs. The amplification and sequencing of the chloroplast inverted repeat B (IRB) region spanning 25,960. bp from P. cerasifera (myrobalan plum) Ehrh., P. amygdalus (almond) and P. persica (peach) using the ASAP method revealed a single nucleotide polymorphisms (SNP) in the rps19. rpl2 IRB region in myrobalan when compared to almond and peach. In addition, a prominent and an easily identifiable single feature polymorphism (SFP-InDel (deletion)) of 18 nucleotides was discovered in reference to the peach chloroplast genome in the ycf1 gene in the IRB region. In this work, it has been developed a highly useful polymorphic molecular marker to characterize the maternal parent in interspecific hybrids of Prunus rootstocks as a first step toward developing pedigree information. The ycf1 SFP-InDel (deletion) has been successfully used in several 3-way hybrids generated in the stone fruit rootstock breeding program for the characterization of new interspecific plant material. This SFP is expected to be highly utile in characterizing the maternal lineage of Prunus hybrids in other breeding programs. © 2014.en
dc.description.otherASAPen
dc.description.otherRootstocken
dc.description.otherInDelen
dc.description.otherPhylogenetic analysisen
dc.description.otherSFPen
dc.description.otherSNPen
dc.description.statusPublishedes_ES
dc.identifier.citationBielsa, B; Jiwan, D.; Fernandez i Marti, A.; Dhingra, A.; Rubio-Cabetas, M. J. Detection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genome. Scientia Horticulturae, 2014, 168, 108-112
dc.identifier.issn0304-4238
dc.identifier.urihttp://hdl.handle.net/10532/7462
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1016/j.scienta.2014.01.028es_ES
dc.relation.referencesThis work was supported by RTA-011-00089 from the Instituto Nacional de Investigacion y Tecnologia Agraria (INIA) and by the foundation DGA (CONSI+D) for a fellowship to MJR to visit the Department of Horticulture at Washington State University, Pullman, WA, USA. Work in the Dhingra lab was supported by Washington State University Agriculture Center Research Hatch funds to AD. We highly appreciate the comment of Dr R. Socias i Company.es_ES
dc.relation.urihttps://doi.org/10.1016/j.scienta.2014.01.028es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocPrunuses
dc.subject.agrovocGenomases
dc.subject.agrovocCloroplastoes
dc.subject.agrovocPortainjertoses
dc.subject.agrovocAnálisis filogenéticoes
dc.subject.otherASAP
dc.subject.otherDna-Sequences
dc.subject.otherindel
dc.subject.otherL.
dc.subject.otherMarkers
dc.subject.otherMicrosatellites
dc.subject.otherNUCLEAR
dc.subject.otherPhylogenetic Analysis
dc.subject.otherPolymorphisms
dc.subject.otherResistance
dc.subject.otherRootstock
dc.subject.otherROOTSTOCKS
dc.subject.otherRosaceae
dc.subject.otherSFP
dc.subject.otherSnp
dc.titleDetection of SNP and validation of a SFP InDel (deletion) in inverted repeat region of the Prunus species chloroplast genomeen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
5822455.pdf
Size:
515.89 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artículos científicos, técnicos y divulgativos