Detection of peanut (Arachis hypogaea) allergens in processed foods by immunoassay: Influence of selected target protein and ELISA format applied

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dc.bibliographicCitation.endpage307es_ES
dc.bibliographicCitation.stpage300es_ES
dc.bibliographicCitation.titleFood Controlen
dc.bibliographicCitation.volume54es_ES
dc.contributor.authorMonserrat, M.es_ES
dc.contributor.authorSanz, D.es_ES
dc.contributor.authorJuan Esteban, Teresaes_ES
dc.contributor.authorHerrero, A.es_ES
dc.contributor.authorSánchez, L.es_ES
dc.contributor.authorCalvo, M.es_ES
dc.contributor.authorPérez, María D.es_ES
dc.date.accessioned2015-03-19T11:27:57Z
dc.date.available2015-03-19T11:27:57Z
dc.date.issued2015es_ES
dc.description.abstractDirect competitive and sandwich ELISA formats developed to determine Ara h1 and Ara h2 proteins were applied in the detection of peanut in model biscuits prepared with a commercial peanut butter as ingredient. The sandwich format for Ara h2 protein could detect the addition of 2.5% peanut butter, whereas the same format for Ara h1 could not detect 5% added peanut. Direct competitive formats for Ara h1 and Ara h2 proteins could detect the addition of 1% and 0.05% peanut butter, respectively. Therefore, competitive format for Ara h2 was selected to be evaluated by four laboratories, obtaining adequate results in term of repeatability and reproducibility. Results obtained indicate that processing decreased the level of extracted proteins and underestimated the amount of Ara h1 and Ara h2 proteins, the effect being more severe for Ara h1. The selection of the target protein and the ELISA format applied greatly influence the detection of peanut in processed foods.en
dc.description.statusPublishedes_ES
dc.description.versionPeer Reviewed
dc.identifier.citationFood Control, 54, p. 300-307en
dc.identifier.issn0956-7135*
dc.identifier.urihttp://hdl.handle.net/10532/2848
dc.language.isoenes_ES
dc.relation.doihttp://dx.doi.org/10.1016/j.foodcont.2015.01.049es_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/es/*
dc.subject.agrovocAlérgenoses
dc.subject.agrovocTécnicas inmunológicases
dc.subject.agrovocCacahuetees
dc.subject.agrovocAlimentos procesadoses
dc.subject.otherotries_ES
dc.titleDetection of peanut (Arachis hypogaea) allergens in processed foods by immunoassay: Influence of selected target protein and ELISA format applieden
dc.typeJournal Contribution*
dc.type.refereedNon-Refereedes_ES
dc.type.specifiedArticlees_ES

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