A Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in rams

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dc.bibliographicCitation.titleVeterinary Researchen
dc.bibliographicCitation.volume53es_ES
dc.contributor.authorMuñoz Álvaro, Pilar Maríaes_ES
dc.contributor.authorConde Álvarez, Raqueles_ES
dc.contributor.authorAndrés Barranco, Saraes_ES
dc.contributor.authorMiguel López, María Jesús dees_ES
dc.contributor.authorZúñiga Ripa, Amaiaes_ES
dc.contributor.authorAragón Aranda, Beatrizes_ES
dc.contributor.authorSalvador Bescós, Miriames_ES
dc.contributor.authorMartínez Gómez, Estrellaes_ES
dc.contributor.authorIriarte, Maitees_ES
dc.contributor.authorBarberán Pelegrín, Montserrates_ES
dc.contributor.authorVizcaíno, Nieveses_ES
dc.contributor.authorMoriyón Uria, Ignacioes_ES
dc.contributor.authorBlasco Martínez, José Maríaes_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2022-03-09T12:22:16Z
dc.date.available2022-03-09T12:22:16Z
dc.date.issued2022es_ES
dc.description.abstractBrucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. B. melitensis carries a smooth (S) lipopolysaccharide (LPS) with an N-formyl-perosamine O-polysaccharide (O-PS) that is absent in the rough LPS of B. ovis. Their control and eradication require vaccination, but B. melitensis Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once B. melitensis is eradicated or where it never existed, hampering B. ovis control and eradication. To develop a B. ovis specific vaccine, we investigated three Brucella live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CAΔwadB (CO2-independent B. ovis with truncated LPS core oligosaccharide); Rev1::wbdRΔwbkC (carrying N-acetylated O-PS); and H38ΔwbkF (B. melitensis rough mutant with intact LPS core). After confirming their attenuation and protection against B. ovis in mice, were tested in rams for efficacy. H38ΔwbkF yielded similar protection to Rev 1 against B. ovis but Bov::CAΔwadB and Rev1::wbdRΔwbkC conferred no or poor protection, respectively. All H38ΔwbkF vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation B. ovis diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for B. melitensis diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38ΔwbkF is an interesting candidate for the immunoprophylaxis of B. ovis in B. melitensis-free areas.en
dc.description.statusPublishedes_ES
dc.identifier.citationVeterinary Research, vol. 53, (2022)
dc.identifier.urihttp://hdl.handle.net/10532/5780
dc.language.isoenes_ES
dc.relation.doi10.1186/s13567-022-01034-zes_ES
dc.relation.urihttps://doi.org/10.1186/s13567-022-01034-zes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.agrovocBrucelosises
dc.subject.agrovocBrucella ovises
dc.subject.agrovocVacunaes
dc.subject.agrovocOvinoses
dc.titleA Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in ramsen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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