Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry

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dc.bibliographicCitation.issue7es_ES
dc.bibliographicCitation.titleBio-protocolen
dc.bibliographicCitation.volume15es_ES
dc.contributor.authorSantolaria Llacer, Nestor Ibónes_ES
dc.contributor.authorFadón Adrián, Ericaes_ES
dc.contributor.authorRodrigo García, Francisco Javieres_ES
dc.contributor.authorHedhly, Afifes_ES
dc.date.accessioned2025-04-21T10:30:14Z
dc.date.available2025-04-21T10:30:14Z
dc.date.issued2025es_ES
dc.date.updated2025-04-21T10:21:13Z
dc.description.abstractStarch is a carbohydrate widely used in the plant kingdom as a fuel for different physiological processes. While different techniques are available for the quantification of starch stored in seeds and bark tissues, they have hardly been used to quantify starch content in developing flower buds, where starch has been reported to accumulate in different reproductive organs. Here, we detail a quantitative enzymatic method to measure starch concentration in developing flower primordia in sweet cherry (Prunus avium L.). First, starch is enzymatically hydrolyzed to D-glucose, which was then quantified by an enzyme-coupled assay involving hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PD) and spectrophotometric quantification of NADH absorbance at 340 nm. This method is a sensitive, rapid, and affordable protocol specifically optimized for tiny flower buds with low starch content. The technique is revealed to successfully determine starch content in non-freshly harvested samples—frozen and stored at -20 °C or stored in fixatives—allowing a temporal separation of sampling and quantification and making the protocol suitable for high-throughput experimental designs in different fields of plant research.en
dc.description.otherStarch enzymatic assayen
dc.description.otherFlower primordiaen
dc.description.otherHK/G6PD assayen
dc.description.otherNADH spectrophotometric assayen
dc.description.otherSweet cherryen
dc.description.otherTemperate fruit treesen
dc.description.sponsorshipEste trabajo fue financiado por el proyecto de I+D+i PID2020-115473RR-I00, financiado por MICIU/AEI/10.13039/501100011033 y el Gobierno de Aragón – Fondo Social Europeo, ‘El FSE invierte en tu futuro’ [Grupo Consolidado A12–17R].es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationSantolaria, N., Fadón, E., Rodrigo, J., & Hedhly, A. (2025). Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry. BIO-PROTOCOL, 15(1368). https://doi.org/10.21769/BioProtoc.5256
dc.identifier.issn23318325
dc.identifier.urihttp://hdl.handle.net/10532/7583
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.21769/bioprotoc.5256es_ES
dc.relation.urihttps://doi.org/10.21769/bioprotoc.5256es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Spaines_ES
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject.agrovocPrunus aviumes
dc.subject.agrovocÁrboles frutaleses
dc.subject.agrovocAnálisis enzimáticoes
dc.subject.agrovocPrimordios foliareses
dc.subject.agrovocEspectrofotometría de llamaes
dc.subject.otherAnálisis Enzimático
dc.subject.otherÁrboles frutales
dc.subject.otherEspectrofotometría
dc.subject.otherPrimordios Foliares
dc.subject.otherPrunus avium
dc.titleEnzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherryen
dc.typearticle*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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