GFP tagging of Brucella melitensis Rev1 allows the identification of vaccinated sheep
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Date
2019
Authors
Zabalza Baranguá, AnaSan Román, Beatriz
Chacón Díaz, Carlos
Miguel López, María Jesús de
Muñoz Álvaro, Pilar María
Iriarte, Maite
Blasco Martínez, José María
Grilló Dolset, María Jesús
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Abstract
Brucellosis is a worldwide zoonosis causing important economic losses and a public health problem. Small ruminants are the preferred hosts of Brucella melitensis and thus the main source of human infections. Effective control of sheep and goat brucellosis has been achieved in several countries through vaccination with the live-attenuated B. melitensis Rev1 vaccine. However, Rev1 induces a long-lasting serological response that hinders the differentiation between infected and vaccinated animals. A Rev1::gfp strain expressing constitutively the Green Fluorescent Protein (GFP) was built by stable insertion of a mini-Tn7-gfp in the glmS-recG non-codifying chromosomal region. An associated indirect ELISA-GFP was developed to identify anti-GFP antibodies in vaccinated animals. The resulting Rev1::gfp kept the biological properties of the Rev1 reference strain, including residual virulence and efficacy in mice, and was readily distinguished from Rev1 and other Brucella field strains by direct visualization under ultraviolet illumination, fluorescence microscopy and a multiplex PCR-GFP. The Rev1::gfp strain did not elicit anti-GFP antibodies itself in lambs but when applied in combination with recombinant GFP induced an intense and long-lasting (>9 months) anti-GFP serological response readily detectable by the ELISA-GFP. Overall, our results confirm that Rev1 GFP-tagging can be a suitable alternative for identifying vaccinated sheep in infected contexts. This article is protected by copyright. All rights reserved.
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Transboundary and Emerging Diseases, vol. 66, num. 1, (2019)
AGROVOC subjects
BrucellaBrucella melitensis
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Vacuna
Ovinos