Protective role of short-chain fatty acids on intestinal oxidative stress induced by TNF-α
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Date
2024-11-14
Authors
Ferrer Navarro, MiguelBuey, Berta
Grasa, Laura
Mesonero, Jose Emilio
Latorre, Eva
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Netherlands
Typology
texto , revista , artículo , artículo originalAbstract
Inflammatory bowel diseases (IBDs) are driven by an exaggerated inflammatory response, which leads to a marked increase in oxidative stress. This, in turn, exacerbates the inflammatory process and causes significant cellular and tissue damage. Intestinal dysbiosis, a common observation in IBD patients, alters the production of bacterial metabolites, including short-chain fatty acids (SCFAs), which are key by-products of dietary fiber fermentation. While the role of SCFAs in intestinal physiology is still being elucidated, this study aimed to investigate their effects on intestinal oxidative stress, particularly under inflammatory conditions induced by the proinflammatory mediator tumor necrosis factor alpha (TNF-α). The Caco-2/TC7 cell line was employed as an in vitro model of the intestinal epithelium, and the cells were treated with a range of SCFAs, including acetate, propionate, and butyrate. The levels of protein and lipid oxidation were quantified, as well as the activity of antioxidant enzymes. Our findings demonstrate that microbiota-derived SCFAs can effectively mitigate TNF-α-induced oxidative stress by modulating antioxidant enzyme activity. The proinflammatory mediator TNF-α induces lipid peroxidation by inhibiting catalase and glutathione peroxidase activities. SCFAs are able to upregulate antioxidant enzyme activity to restore lipid oxidative levels. These results underscore the critical role of the gut microbiota in maintaining intestinal homeostasis and highlight the therapeutic potential of SCFAs in managing oxidative stress-related pathologies.
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Bibliographic citation
Ferrer, M., Buey, B., Grasa, L., Mesonero, J. E., & Latorre, E. (2024). Protective role of short-chain fatty acids on intestinal oxidative stress induced by TNF-α. Cell Stress and Chaperones, 29(6), 769-776. https://doi.org/10.1016/j.cstres.2024.11.002
AGROVOC subjects
Enfermedad gastrointestinalIntestino
Inflamación
Colitis
Aceite graso
Microbiota
Ácido propiónico
Factor necrosis tumoral
Sponsorship
Fundación Ibercaja-Unizar (JIUZ-2018-BIO-04)