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http://hdl.handle.net/10532/7139
Título : | Genome editing in almond: A CRISPR-based approach through hairy root transformation |
Autor : | Jedličková, Veronika Štefková, Marie Sánchez López, Juan Francisco Grimplet, Jérôme Rubio Cabetas, María José Robert, Hélène S. |
Fecha de publicación : | 2024 |
Citación : | Biorxiv, 2024 |
Resumen : | Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technology has revolutionized genome manipulation for crop enhancement, providing a powerful toolkit. However, the tissue culture and plant regeneration steps that are critical to the CRISPR/Cas editing framework are often challenging, especially in some woody plant species that exhibit substantial resistance to these procedures. To address this, we have developed an injection-based protocol for inducing hairy roots in almond (Prunus dulcis, syn. Prunus amygdalus), a species known for its recalcitrance to conventional transformation methods. Notably, the hairy root induction method also proved effective in almond x peach hybrids. To evaluate its utility for gene functional analysis, we combined the hairy root transformation system with CRISPR/Cas9 gene editing technology, targeting two transcription factor genes (ERF74 and GAI). Our efforts resulted in transformants with target knock-out, suggesting the potential of this genetic transformation technology as a valuable tool for future routine gene function studies in almond. |
URI : | http://hdl.handle.net/10532/7139 |
Documento relativo: | https://doi.org/10.1101/2024.04.11.588989 |
Licencia: | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Aparece en las colecciones: | [DOCIART] Artículos científicos, técnicos y divulgativos |
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2024.04.11.588989v1.pdf | 327,7 kB | Adobe PDF | ![]() Visualizar/Abrir |
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