Ovine oocytes display a similar germinal vesicle configuration and global DNA methylation at prepubertal and adult ages
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Date
2019
Authors
Cocero, M.Marigorta, P.
Novillo, F.
Folch Pera, José
Sánchez Ruiz, Pilar
Alabart Alvarez, José Luis
Lahoz Crespo, Belén
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Abstract
© 2019 Elsevier Inc. Epigenetic mechanisms are thought to be involved in the reduced developmental capacity of early prepubertal ewe oocytes compared to their adult counterparts. In this study, we have analyzed the global DNA methylation pattern and in vitro meiotic and developmental competence of oocytes at the germinal vesicle (GV) stage obtained from adult and 3-month-old donors. All oocytes were aspirated from antral follicles with a diameter ≥3 mm, and DNA methylation on 5-methylcytosine was detected by immunofluorescence using an anti-methyl cytosine antibody. The main global chromatin configuration pattern shown by both prepubertal and adult ovine oocytes corresponded to condensed chromatin localized close to the nuclear envelope (the SNE pattern). Immunofluorescence showed that a global bright nuclear staining of 5-methylcytosine (5-mC) occurred in all germinal vesicle stage oocytes and matched the propidium iodide staining pattern. The total fluorescence intensity values of lamb GVs were not lower than those observed in adult GVs. The meiotic competence and cleavage rates were similar in adult and prepubertal oocytes, however, the developmental competence of embryos to reach blastocysts was higher for adult oocytes than lamb oocytes (p<0.0001). In conclusion, our results indicate that adult-size oocytes derived from 3 to 4 month old prepubertal ewes show similar GV morphology and DNA methylation staining patterns to those obtained from adult animals, despite exhibiting a lower developmental competence.
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Bibliographic citation
Cocero, Maria J; Marigorta, P.; Novillo, F.; Folch, J.; Sanchez, P.; Alabart, J. L; Lahoz, B. Ovine oocytes display a similar germinal vesicle configuration and global DNA methylation at prepubertal and adult ages. Theriogenology, 2019, 138, 154-163
AGROVOC subjects
OvocitosOveja
Epigenético
Blastocistos
Cromatina
Other field subjects
Antral follicleBlastocyst
Chromatin
CHROMATIN CONFIGURATION
Developmental competence
Embryo
Epigenetic
Juvenile
maturation
Morphology
OOGENESIS
Organization
Progression
sheep
SHEEP OOCYTES
Age Factors
Aging
Animals
antral follicle
BLASTOCYST
Cell Nucleus
Chromatin
Dna Methylation
Embryo
Epigenesis, Genetic
Epigenetic
Female
In Vitro Oocyte Maturation Techniques
In-Vitro
Meiosis
Oocyte Donation
Oocytes
sexual maturation
Sheep
Sponsorship
This work was funded was suportad by INIA, Spanish Ministry
of Economy and Competitiveness (RTA2011-00128-C02 and
RTA2013-00041-C02)